Ab47720

The antibody against inhibin‐α (R1, ab14087, Abcam, Cambridge, United Kingdom) was used for immunohistochemistry and immunofluorescence analyses (dilution at 1:200). The antibody against inhibin‐α (4A2F2, ab47720, Abcam) was used for immunoblotting (dilution at 1:500) and flow cytometry analyses (1 µg for 10⁶ cells.). The antibody against ALDH1A1 (D9Q8E, 54135, Cell Signaling Technology, Inc., Danvers) was used for immunohistochemistry (dilution at 1:2000) and immunoblotting (dilution at 1:1000) analyses. Antibody for immunohistochemistry against Ki‐67 (MIB‐1, M7240, dilution at 1:100) was obtained from Dako/Agilent Technologies, Inc. (Santa Clara). Antibody for immunoblotting against β‐actin (horseradish peroxidase [HRP] conjugate) (13E5, 5125, dilution at 1:1000) was obtained from Cell Signaling Technology, Inc. The antibody against vascular endothelial growth factor (VEGF)‐A (VG‐1, ab1316, Abcam) was used for immunohistochemistry and immunoblotting (dilution at 1:200). The antibody against VEGF‐C (9/E7, ab106512, Abcam) was used for immunohistochemistry and immunoblotting (dilution at 1:400).

Immunofluorescence (IF) was performed on sectioned paraffin-embedded tumor constructs or fixed cells cultured for 3–7 days on X‐tra^® slides to analyze biomarker presence of SF-1 (ab240394, Abcam), Melan A (ab210546, Abcam), inhibin-α (ab47720, Abcam), IGF-2 (ab226989, Abcam), and β-catenin (ab ab223075, Abcam). Nuclei were stained with DAPI (P36962, ThermoFisher). The slides were imaged with Nikon A1R confocal microscope at 40 × magnification at room temperature.