St7l antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The ST7L antibody is a research-use-only reagent produced by Santa Cruz Biotechnology. It is designed to detect the ST7L (suppression of tumorigenicity 7 like) protein. The ST7L protein is involved in cellular processes, but its precise function is not fully characterized. The antibody can be used in various research applications to study the ST7L protein, such as western blotting, immunoprecipitation, and immunohistochemistry, among others.

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Lab products found in correlation

Bs 0295g cy3, by Bioss Antibodies (1 mentions) Dmi4000b microscope, by Leica (1 mentions) Pan akt antibody, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Ki67 antibody, by Abcam (1 mentions)

2 protocols using st7l antibody

Immunofluorescence Analysis of AKT and ST7L

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Cells cultured on glass coverslips were fixed with 4% paraformaldehyde, blocked with 5% bovine serum albumin and incubated with AKT (pan) antibody (no.4691; Cell Signaling Technology) and ST7L antibody (sc-138649; Santa Cruz, Biotechnology) overnight at 4 °C. Then, cells were incubated with mouse anti-goat IgG/FITC antibody (bs-0294M-FITC, BIOSS) and goat anti-rabbit IgG/Cy3 antibody (bs-0295G-Cy3, BIOSS) for 2 h at room temperature. DAPI (Invitrogen) was used to stain the nucleus. LEICA DMI4000B microscope (Leica, Heidelberg, Germany) was used to take the images.

Zhuang L., Wang X., Wang Z., Ma X., Han B., Zou H., Wu Z., Dong S., Qu Z., Zang Y, & Wu L. (2017). MicroRNA-23b functions as an oncogene and activates AKT/GSK3β/β-catenin signaling by targeting ST7L in hepatocellular carcinoma. Cell Death & Disease, 8(5), e2804-.

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Immunohistochemical Analysis of Tumor Tissues

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Immunostaining was performed on the paraffin-embedded tumor tissues from nude mice. ST7L antibody (1:200, Santa Cruz, CA, USA) and Ki67 antibody (1:500, Abcam, Cambridge, UK) were used in immunohistochemistry with the method of streptavidin-peroxidase conjugated. Sections were visualized under a microscope at 400 × or 200 × (ZEISS, Germany).

Yan S., Tang Z., Chen K., Liu Y., Yu G., Chen Q., Dang H., Chen F., Ling J., Zhu L., Huang A, & Tang H. (2018). Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression. Journal of Experimental & Clinical Cancer Research : CR, 37, 214.

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