For flow cytometry-based sorting of infected cells, mice were infected with 104 PFU MHV68-H2bYFP, a phenotypically wild-type virus that expresses eYFP under control of the H2b promoter [44 (link)]. At 16 dpi, splenocytes were prepared and blocked as described above. Cells were then stained with APC rat anti-mouse CD4 at 1:200 (BD Biosciences, 553051), APC rat anti-mouse CD8α at 1:200 (BD Biosciences, 553035), APC rat anti-mouse CD14 at 1:100 (BD Biosciences, 560634), and APC-Cy7 rat anti-mouse CD19 at 1:200 (BD Biosciences, 557655). Infected B cells (CD4- CD8- CD14- CD19+ YFP+ ) and non-infected B cells (CD4- CD8- CD14- CD19+ YFP- ) were sorted using a BD FACSAria II flow cytometer (BD Biosciences). Sorted cells were immediately subjected to RNA extraction using an RNAqueous-Micro kit (Ambion, AM1931) prior to qRT-PCR analyses.
Apc rat anti mouse cd8α
Manufactured by BD
The APC rat anti-mouse CD8α is a laboratory reagent used for the detection and analysis of CD8α-positive cells in mouse samples. It is a monoclonal antibody conjugated with the fluorescent dye Allophycocyanin (APC), which allows for the visualization and quantification of CD8α-expressing cells through flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Rnaqueous micro kit, by Thermo Fisher Scientific (1 mentions)
Apc rat antimouse cd4, by BD (1 mentions)
Apc cy7 rat anti mouse cd19, by BD (1 mentions)
Facsaria 2, by BD (1 mentions)
Pe rat anti mouse siglec f, by BD (1 mentions)
Apc cy 7 rat anti mouse cd4, by BD (1 mentions)
Pe mouse anti mouse nk 1, by BD (1 mentions)
Apc cy 7 rat anti cd11b, by BD (1 mentions)
F4 80 monoclonal antibody apc, by Thermo Fisher Scientific (1 mentions)
Pe rat anti mouse cd14, by BD (1 mentions)
Fitc anti mouse cd11c antibody, by BioLegend (1 mentions)
Facscanto 2, by BD (1 mentions)
2 protocols using apc rat anti mouse cd8α
1
Flow Cytometry-based Sorting of Infected Cells
2
Flow Cytometric Analysis of Immune Cells
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Cell suspensions were obtained from BALF and were washed and resuspended in PBS-1 %BSA before FASC analysis. All flow cytometry data was acquired using FACS Canto Ⅱ (BD) and data was analyzed using FlowJo 10.4. The following antibodies were used in flow cytometric analyses: BV421 Hamster Anti-Mouse TCR β Chain (BD Horizon, catalog no. 562839), APC-Cy™7 Rat Anti-Mouse CD4 (BD Pharmingen, catalog no. 565650), APC Rat Anti-Mouse CD8α (BD Pharmingen, catalog no. 553035), PE Mouse Anti-Mouse NK-1.1 (BD Pharmingen, catalog no. 557391), APC-Cy™7 Rat Anti-CD11b (BD Pharmingen, catalog no. 557657), FITC anti-mouse CD11c Antibody (Biolegend, catalog no. 117306), F4/80 Monoclonal Antibody APC (eBioscience, catalog no. 17-4801-80), PE Rat anti-Mouse CD14 (BD Pharmingen, catalog no. 553740), PE Rat Anti-Mouse Siglec-F (BD Pharmingen, catalog no. 552126).
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