Molecular weights and PDIs were measured by Waters gel permeation chromatography in THF relative to polymethylmethacrylate (PMMA) as well as polystyrene (PS) standards on systems equipped with Waters Model 515 HPLC pump and Waters Model 2414 Refractive Index Detector at 35 °C with a flow rate of 1 mL/min. HRMS analyses were performed with Q-TOF YA263 high resolution (Waters Corporation) instruments by +ve mode electrospray ionization.
Model 515 hplc pump
Manufactured by Waters Corporation
The Waters Model 515 HPLC pump is a high-performance liquid chromatography pump designed for reliable and precise solvent delivery. It features a constant flow rate across a wide range of operating pressures and supports a maximum operating pressure of 6000 psi. The pump is suitable for use in a variety of HPLC applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using model 515 hplc pump
1
Polymer Molecular Weight Analysis
2
Polymer-Mediated Bacterial Characterization
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GPC measurements were conducted in
tetrahydrofuran at 30 °C with a flow rate of 1.0 mL/min (equipped
with a Waters Model 515 HPLC pump, one PolarGel-M guard column, and
two PolarGel-M analytical columns (300 × 7.5 mm2)).
Detection consisted of a Waters Model 2414 RI detector. Narrow molecular
weight poly(methyl methacrylate) (PMMA) standards (Mp values ranging from 1280 to 199 000 g/mol) were
used to calibrate the GPC system. NMR spectra were acquired in a Bruker
AvanceIII 500 MHz spectrometer at 25 °C. Gram staining
images of bacteria were taken using an optical microscope at 40×
and 100× resolution before and after polymer treatment. Optical
density (OD) measurements of bacterial solutions with and without
polymer at 600 nm (OD600) were performed using a Hitachi
U2900 spectrometer.
tetrahydrofuran at 30 °C with a flow rate of 1.0 mL/min (equipped
with a Waters Model 515 HPLC pump, one PolarGel-M guard column, and
two PolarGel-M analytical columns (300 × 7.5 mm2)).
Detection consisted of a Waters Model 2414 RI detector. Narrow molecular
weight poly(methyl methacrylate) (PMMA) standards (Mp values ranging from 1280 to 199 000 g/mol) were
used to calibrate the GPC system. NMR spectra were acquired in a Bruker
AvanceIII 500 MHz spectrometer at 25 °C. Gram staining
images of bacteria were taken using an optical microscope at 40×
and 100× resolution before and after polymer treatment. Optical
density (OD) measurements of bacterial solutions with and without
polymer at 600 nm (OD600) were performed using a Hitachi
U2900 spectrometer.
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