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2 protocols using thymidine 5 monophosphate disodium salt

1

Enzymatic Assay for Folate Metabolism

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Chemicals and reagents 2-(4-chlorophenyl)ethylamine, ethyl chloroformate (ECF), diethyl ether, ethyl acetate, dichloromethane and thymidine 5′-monophosphate disodium salt were obtained from Sigma-Aldrich. Tetrahydrofolate and (6S)-5-formyl tetrahydrofolate were kindly provided by Merck & Cie (Schaffhausen, Switzerland). Tritiated glycine used in the radioisotopic assay ([2−3H]glycine) was purchased from Perkin Elmer. (6R,S)-5-Formyl-5,6,7,8-tetrahydropteroylpenta-γ-L-glutamic acid lithium salt (5-CHO-THF-Glu5) was purchased from Schircks Laboratories, Bauma, Switzerland. All other reagents used in the enzymatic assays were obtained from Sigma-Aldrich.
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2

Fabrication of Microfluidic Devices

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Chemicals and materials were obtained from the following sources and used without further purification: 2'-Deoxyadenosine-5'-monophosphate (Alfa Aesar); Thymidine-5'-monophosphate disodium salt (Alfa Aesar); 2'-Deoxycytidine-5'-monophosphate (Alfa Aesar); 2'-Deoxyguanosine-5'-monophosphate disodium salt hydrate (Alfa Aesar); potassium chloride (1 and 3 m, Sigma-Aldrich); Tris-EDTA (100×, Sigma-Aldrich); Tris-Borate-EDTA (10×, Sigma-Aldrich); silver (Ag) wires (>99%, Sigma-Aldrich); S1813 photoresist (MicroChem); MF319 developer (MicroChem); potassium hydroxide (KOH) pellets (Fisher Scientific); hydrofluoric acid (HF) (Sigma-Aldrich); Fluorolink MD 700 (Solvay); 2-hydroxy-2-methylpropiophenone (Sigma-Aldrich); NOA72 (Norland Products); poly(ethylene glycol) diacrylate (PEGDA) (Mn = 250, Sigma-Aldrich); 2,2-dimethoxy-2-phenylacetophenone (Sigma-Aldrich); Si wafers (P/B, resistivity 5-10 Ωcm, orientation of (100), and 525 ± 25 μm thickness) (WaferPro); polyethylene terephthalate (PET) with 250 μm thickness (Goodfellow); polymethylmethacrylate (PMMA) with a thickness of 250 μm (Goodfellow); polymethylmethacrylate (PMMA) of 1 mm thickness (ePlastics).
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