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Vδ1 fitc clone ts8

Manufactured by Thermo Fisher Scientific
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Vδ1-FITC (clone TS8.2) is a fluorescently labeled monoclonal antibody that binds to the Vδ1 chain of the T cell receptor on human T cells. This product is intended for use in flow cytometry applications to identify and quantify Vδ1+ T cells in biological samples.

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Lab products found in correlation

Cd4 microbeads, by Miltenyi Biotec (1 mentions) Facsdiva software, by BD (1 mentions) Fixable viability dye efluor 506, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Hucd45 pb clone hi30, by Sony (1 mentions) Pan γδtcr pe clone immu510, by Beckman Coulter (1 mentions) Mcd45 apc clone 30 f11, by Sony (1 mentions) αβtcr fitc clone ip26, by BioLegend (1 mentions) Cd8 percpcy5.5 clone rpa t8, by BioLegend (1 mentions)

Spelling variants of this product

Anti–Vδ1 TCR FITC clone TS8.2 (2 citations)

2 protocols using vδ1 fitc clone ts8

1

CD4+ T-Cell Enrichment and Expansion Protocol

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The separation of CD4+ TEGs was performed using CD4 Microbeads (Miltenyi Biotech) as per the manufacturer’s instructions. Briefly, TEGs that were previously expanded on REP were incubated with magnetic microbeads cells and loaded into LS column for MACS cell separation. Thereafter, CD4+ selected or bulk (with CD4:CD8 ratio 50:50) TEGs were expanded separately on the next REP cycle prior to in vitro functional assay. TEG expression was monitored prior to functional assays or in vivo infusion by flow cytometry using anti-αβTCR-APC (clone IP26, eBioscience), anti-pan-γδTCR-PE (clone IMMU510, Beckman Coulter), anti-CD8-PerCP-Cy5.5 (clone RPA-T8, Biolegend), anti-CD4-PeCy7 (clone TPA-R4, Biolegend), anti-CD4-FITC (clone TPA-R4, Biolegend), and Vδ1-FITC (clone TS8.2, Thermo Fisher Scientific) antibodies.
Johanna I., Hernández-López P., Heijhuurs S., Scheper W., Bongiovanni L., de Bruin A., Beringer D.X., Oostvogels R., Straetemans T., Sebestyen Z, & Kuball J. (2021). Adding Help to an HLA-A*24:02 Tumor-Reactive γδTCR Increases Tumor Control. Frontiers in Immunology, 12, 752699.
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2

Multiparametric Flow Cytometry Analysis

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The following antibodies were used for flow cytometry analysis: huCD45-PB (clone HI30; Sony Biotechnology, Surrey, United Kingdom), mCD45-APC (clone 30-F11, Sony Biotechnology), αβTCR-FITC (clone IP26; Biolegend, London, United Kingdom), pan-γδTCR-PE (clone IMMU510; Beckman Coulter, Woerden, The Netherlands), CD8-PerCPCy5.5 (clone RPA-T8, Biolegend), CD4-PeCy7 (clone TPA-R4, Biolegend), and Vδ1-FITC (clone TS8.2, Thermo Fisher Scientific, Breda, The Netherlands). To exclude nonviable cells from the analysis, Fixable Viability Dye eFluor506 was used (eBioscience, Thermo Fisher Scientific). All samples were analyzed on a BD LSRFortessa using FACS-Diva Software (BD Biosciences).
Johanna I., Hernández-López P., Heijhuurs S., Bongiovanni L., de Bruin A., Beringer D., van Dooremalen S., Shultz L.D., Ishikawa F., Sebestyen Z., Straetemans T, & Kuball J. (2020). TEG011 persistence averts extramedullary tumor growth without exerting off-target toxicity against healthy tissues in a humanized HLA-A*24:02 transgenic mice. Journal of leukocyte biology, 107(6), 1069-1079.
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