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Bio plex pro 200 system

Manufactured by Bio-Rad
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The Bio-Plex Pro 200 system is a multiplex assay platform that enables the simultaneous detection and quantification of multiple analytes in a single sample. The system utilizes magnetic beads coated with specific capture antibodies to bind and measure target proteins or other molecules of interest.

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Lab products found in correlation

Lipofectamine messengermax, by Thermo Fisher Scientific (2 mentions) Bio plex pro human inflammation panel 1, by Bio-Rad (2 mentions) Poly d lysine coated cell culture plate, by Greiner (2 mentions)

Close spelling variants of this product

Bio-Plex 200 Bio-Plex system Bio-Plex

2 protocols using bio plex pro 200 system

1

IFN-β Secretion Assay in BJ Fibroblasts

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BJ fibroblasts were seeded in 96-well poly-d-lysine-coated cell culture plates (Greiner) at 20 000 cells per well and transfected the next day with 50 ng per well of the respective mRNA using Lipofectamine™ MessengerMAX™ (Life Technologies). The supernatant was harvested 48 h post-transfection and interferon-β (IFN-β) levels were determined using the Bio-Plex Pro Human Inflammation Panel 1 (BioRad) as per the manufacturer's protocol. Data were acquired on the Bio-Plex Pro 200 system (BioRad).
Vostrosablin N., Lim S., Gopal P., Brazdilova K., Parajuli S., Wei X., Gromek A., Prihoda D., Spale M., Muzdalo A., Greig J., Yeo C., Wardyn J., Mejzlik P., Henry B., Partridge A.W, & Bitton D.A. (2024). mRNAid, an open-source platform for therapeutic mRNA design and optimization strategies. NAR Genomics and Bioinformatics, 6(1), lqae028.
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2

IFN-β Secretion Assay in BJ Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
BJ fibroblasts were seeded in 96-well poly-d-lysine-coated cell culture plates (Greiner) at 20 000 cells per well and transfected the next day with 50 ng per well of the respective mRNA using Lipofectamine™ MessengerMAX™ (Life Technologies). The supernatant was harvested 48 h post-transfection and interferon-β (IFN-β) levels were determined using the Bio-Plex Pro Human Inflammation Panel 1 (BioRad) as per the manufacturer's protocol. Data were acquired on the Bio-Plex Pro 200 system (BioRad).
Vostrosablin N., Lim S., Gopal P., Brazdilova K., Parajuli S., Wei X., Gromek A., Prihoda D., Spale M., Muzdalo A., Greig J., Yeo C., Wardyn J., Mejzlik P., Henry B., Partridge A.W, & Bitton D.A. (2024). mRNAid, an open-source platform for therapeutic mRNA design and optimization strategies. NAR Genomics and Bioinformatics, 6(1), lqae028.
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