Analytical-grade reagents were purchased from Sigma-Aldrich unless stated otherwise. Antibiotics were from Sigma-Aldrich, Invivogen or Invitrogen. Other reagents/materials were obtained as follows: D-2-[3H]-mannose (20–30 Ci/mmol) (American Radiolabeled Chemicals Inc.), Micro BCA protein assay kit (Thermo Fisher Scientific), Triton X-100 (Roche), IgG-Agarose resin (GE Healthcare), anti-TAP antibody (Genescript), anti-Dpm1 antibody (Abcam), SM-2 Biobeads and P6-Biogel resin (Biorad) and egg phosphatidylcholine (Avanti Polar Lipids). Yeast strains used in this study are listed in Table S5 .
Igg agarose resin
Manufactured by GE Healthcare
IgG-Agarose resin is a chromatography medium used for the purification of immunoglobulin G (IgG) antibodies from various sample sources. The resin is composed of agarose beads coupled with protein A or protein G, which have a high affinity for the Fc region of IgG molecules. This allows for the selective capture and separation of IgG from complex mixtures, such as cell culture supernatants or serum samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti dpm1 antibody, by Abcam (2 mentions)
Sm 2 biobeads and p6 biogel resin, by Bio-Rad (2 mentions)
Micro bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Egg phosphatidylcholine, by Avanti Polar Lipids (2 mentions)
Triton x 100, by Roche (2 mentions)
Analytical grade reagents, by Merck Group (1 mentions)
2 protocols using igg agarose resin
1
Cell Surface Glycan Labeling Protocol
2
Yeast Protein Purification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Analytical-grade reagents were purchased from Sigma-Aldrich unless stated otherwise.
Antibiotics were from Sigma-Aldrich, Invivogen or Invitrogen. Other reagents/materials were obtained as follows: D-2-[ 3 H]-mannose (20-30 Ci/mmol) (American Radiolabeled Chemicals Inc.), Micro BCA protein assay kit (Thermo Fisher Scientific), Triton X-100 (Roche), IgG-Agarose resin (GE Healthcare), anti-TAP antibody (Genescript), anti-Dpm1 antibody (Abcam), SM-2 Biobeads and P6-Biogel resin (Biorad) and egg phosphatidylcholine (Avanti Polar Lipids). Yeast strains used in this study are listed in Table S5.
Antibiotics were from Sigma-Aldrich, Invivogen or Invitrogen. Other reagents/materials were obtained as follows: D-2-[ 3 H]-mannose (20-30 Ci/mmol) (American Radiolabeled Chemicals Inc.), Micro BCA protein assay kit (Thermo Fisher Scientific), Triton X-100 (Roche), IgG-Agarose resin (GE Healthcare), anti-TAP antibody (Genescript), anti-Dpm1 antibody (Abcam), SM-2 Biobeads and P6-Biogel resin (Biorad) and egg phosphatidylcholine (Avanti Polar Lipids). Yeast strains used in this study are listed in Table S5.
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