P27kip1 sc 528

Manufactured by Santa Cruz Biotechnology

Sourced in United States

P27Kip1 (sc-528) is a primary antibody product developed by Santa Cruz Biotechnology. It is a mouse monoclonal antibody that recognizes the P27Kip1 protein, which is a member of the Cip/Kip family of cyclin-dependent kinase (CDK) inhibitors.

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Lab products found in correlation

Doxercalciferol, by Merck Group (1 mentions) Carnosic acid, by Enzo Life Sciences (1 mentions) Phospho chk1 2348, by Cell Signaling Technology (1 mentions) Arabinocytosine, by Merck Group (1 mentions) Pan akt, by Cell Signaling Technology (1 mentions) Pakt t308, by Cell Signaling Technology (1 mentions) Ps6 s235 236, by Cell Signaling Technology (1 mentions) Hsp90 alpha beta, by Santa Cruz Biotechnology (1 mentions) Alexa680 coupled secondary antibodies, by Thermo Fisher Scientific (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Hpa001758, by Merck Group (1 mentions) Lsm 780 nlo inverted axio observer z1 confocal microscope, by Zeiss (1 mentions) Axiocam mrm camera, by Zeiss (1 mentions) Axio imager z1 microscope, by Zeiss (1 mentions) Ecl method, by Cytiva (1 mentions) Jak2 clone d2e12, by Cell Signaling Technology (1 mentions) Gapdh clone 14c10, by Cell Signaling Technology (1 mentions)

4 protocols using p27kip1 sc 528

Molecular Mechanisms of Carnosic Acid

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Arabinocytosine and Doxercalciferol were purchased from Sigma-Aldrich (St. Louis, MO). Carnosic acid (CA) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY). The following antibodies: Crk-L (sc-319), VDR (sc-1008), C/EBP beta (sc-150), and p27Kip1 (sc-528) were obtained from Santa Cruz Biotechnology (Dallas, TX). Phospho-Ser139-H2AX (#9718), phospho-Chk1 (#2348), and HRP-linked anti-rabbit (#7074) antibodies were purchased from Cell Signaling Technologies (Danvers, MA).

Wang X., Harrison J.S, & Studzinski G.P. (2015). Enhancement of Arabinocytosine (AraC) toxicity to AML cells by a differentiation agent combination. The Journal of steroid biochemistry and molecular biology, 164, 72-78.

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Western Blot Analysis of AKT and S6 Signaling

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Whole-cell lysates were prepared and isolated proteins separated in SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes and incubated at 4°C overnight with the following primary antibodies: pAKT-T308 (#2965, RRID:AB_2255933), pAKT-S473 (#4060, RRID:AB_2315049), pan-AKT (#4691, RRID:AB_915783), p-S6-S235/236 (#4858, RRID:AB_916156; all from Cell Signaling Technology) and p27^Kip1 (sc528, RRID:AB_632129), Skp2 (sc7164, RRID:AB_2187650) and Hsp90 alpha/beta (sc13119, RRID:AB_675659; all from Santa Cruz Biotechnology). Primary antibodies were followed by Alexa680-coupled secondary antibodies (Thermo Fisher Scientific) and detected by the Odyssey Infrared Imaging System (Licor, Bad Homburg, Germany).

Falcomatà C., Bärthel S., Ulrich A., Diersch S., Veltkamp C., Rad L., Boniolo F., Solar M., Steiger K., Seidler B., Zukowska M., Madej J., Wang M., Öllinger R., Maresch R., Barenboim M., Eser S., Tschurtschenthaler M., Mehrabi A., Roessler S., Goeppert B., Kind A., Schnieke A., Robles M.S., Bradley A., Schmid R.M., Schmidt-Supprian M., Reichert M., Weichert W., Sansom O.J., Morton J.P., Rad R., Schneider G, & Saur D. (2021). Genetic Screens Identify a Context-Specific PI3K/p27Kip1 Node Driving Extrahepatic Biliary Cancer. Cancer Discovery, 11(12), 3158-3177.

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Immunohistochemical Analysis of Gonad Development

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Gonad samples were fixed with 4% paraformaldehyde overnight, processed for paraffin embedding, and sectioned at 5 μm thick. The following dilutions of primary antibodies were used: AMH/MIS (c-20, sc-6886, Santa Cruz), 1:200; DMRT1 (HPA027850, Sigma), 1:100; FOXL2 (NB100-1277, Novus), 1:200; GATA1 (N6, sc-265, Santa Cruz), 1:200; GATA4 (C20, sc-1237, Santa Cruz), 1:200; 3βHSD (P18, sc-30820, Santa Cruz), 1:200; P27 (Kip1, sc-528, Santa Cruz), 1:200; LAMA1 (L9393, Sigma), 1:150; SF1 (kindly provided by Ken Morohashi), 1:1000; SOX8 (kindly provided by Elisabeth Sock [Stolt et al., 2005 (link)]), 1:1000; SOX9 (HPA001758, Sigma), 1:200; and TRA98 (ab82527, Abcam), 1:200. Counterstain with DAPI was used to detect nuclei. Immunofluorescence of secondary antibodies were detected with an Axio ImagerZ1 microscope (Zeiss) coupled to an Axiocam mrm camera (Zeiss) or a LSM 780 NLO inverted Axio Observer.Z1 confocal microscope (Carl Zeiss Microscopy GmbH, Jena,Germany) using a Plan Apo 10X dry NA 0.45 objective. Images were processed with Axiovision LE and Serif Affinity Photo software. Immunostaining experiments were performed on gonads from at least three mice for each genotype.

Richardson N., Gillot I., Gregoire E.P., Youssef S.A., de Rooij D., de Bruin A., De Cian M.C, & Chaboissier M.C. (2020). Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals. eLife, 9, e53972.

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Protein Expression Analysis by Western Blot

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Proteins were fractionated by electrophoresis, electroblotted to nitrocellulose membrane (PROTRAN, Whatman Dassel, Germany) and probed with antibodies against STAT5a/b (cat. n. #9363), JAK2 (clone D2E12), phosphorylated STAT5 (Tyr684, clone D47E7), phospho-JAK2 (Tyr1007/1008, clone C80C3), GAPDH (clone 14C10) (Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (sc-182) and p27^Kip1 (sc-528) (Santa Cruz Biotechnology, Dallas, TX, USA). Immunoreactivity was determined using the ECL method (Amersham Biosciences, Little Chalfont, UK), according to manufacturer’s instruction. Full-length gels and blots are included in the Supplementary Materials (Figures S1–S9).

De Marinis E., Ceccherelli A., Quattrocchi A., Leboffe L., Polticelli F., Nervi C, & Ascenzi P. (2019). Ruxolitinib binding to human serum albumin: bioinformatics, biochemical and functional characterization in JAK2V617F+ cell models. Scientific Reports, 9, 16379.

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