Blood was collected from the tail vein of mice fasted overnight. Whole blood was used to measure blood glucose by a ContourNext glucometer (Bayer) and hemoglobin A1c (Crystal Chem) per manufacturer's instructions. Remaining blood samples were centrifuged to separate the plasma, and plasma insulin levels were measured by UltraSensitive Mouse Insulin ELISA kit (Crystal Chem), according to manufacturer's instructions. The homeostasis model assessment of insulin resistance index was calculated using the formula: (fasting blood glucose [mg/dL]×fasting plasma insulin [μU/mL])÷405.
Hemoglobin a1c
Manufactured by Crystal Chem
The Hemoglobin A1c is a lab equipment used to measure the average level of blood sugar over a 2-3 month period. It provides a quantitative assessment of glucose control.
Automatically generated - may contain errors
2 protocols using hemoglobin a1c
1
Comprehensive Metabolic Profiling in Mice
2
Comprehensive Metabolic Profiling of Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercially-available colorimetric assay kits were used to measure the activity and/or concentration in whole blood or blood plasma of each of the following: Hemoglobin A1c (Catalog #80,310, Crystal Chem, Elk Grove Village); alanine aminotransferase (ALT) activity (Cat#MAK052, MilliporeSigma, St. Louis);, aspartate aminotransferase (AST) activity (Cat#MAK055, MilliporeSigma), triglycerides (Cat#100,103,003, Cayman Chemical), and urea concentration (Cat#MAK006). Plasma adiponectin (Cat#80,569), C-Peptide (Cat#90,050), insulin (Cat#90,080), and leptin (Cat#90,030) concentrations were assessed by ELISA (Crystal Chem). All samples were measured in triplicate and samples from a minimum of three mice were measured per study timepoint.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!