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Tcs sp5 laser scanning microscopy

Manufactured by Leica
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The TCS Sp5 Laser Scanning Microscopy is a high-performance confocal microscope system designed for advanced imaging applications. It utilizes a combination of laser illumination and precision scanning to capture detailed images of microscopic samples. The system features a modular design, allowing for customization and adaptation to specific research requirements.

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Lab products found in correlation

Biotin blocking kit, by Vector Laboratories (2 mentions) Prolong anti fade reagents, by Thermo Fisher Scientific (2 mentions) Cm1850, by Leica (1 mentions) Tissue tek optimum cutting temperature compound, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TCS SP5 (2664 citations) TCS-SP5 laser scanning confocal microscopy (15 citations) Scanning laser microscopy (2 citations)

2 protocols using tcs sp5 laser scanning microscopy

1

Multicolor Immunofluorescence Staining of Lymph Node

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Whole tissue (popliteal lymph node or hepatic lymph node) was collected and placed in Tissue-Tek optimum cutting temperature compound (OCT) (Thermo Scientific) and frozen in liquid nitrogen. Serial cryostat sections (10μm) were collected using a Leica CM 1850. Sections were then air-dried and fixed in ice-cold 75% acetone/25% ethanol for 5 mins. Sections were rehydrated in PBS for 5 to 10 minutes and blocked using a biotin blocking kit (Vector Laboratories) followed by incubation with 1%(v/v) in PBS of rat and rabbit serum. Staining with appropriate antibodies was done overnight at 4°C followed by secondary staining for 1 hour at room temperature. Coverslips were mounted using ProLong anti-fade reagents (Life Technologies). Images were acquired with a Leica TCS Sp5 Laser Scanning Microscopy with an average grid size of 3x3. Images were taken with a 20x objective at a resolution of 1024x1024. Image post-processing was done using Fiji is Just ImageJ software (1.47v).
Cortés-Selva D., Gibbs L., Ready A., Ekiz H.A., O’Connell R., Rajwa B, & Fairfax K.C. (2021). Maternal schistosomiasis impairs offspring Interleukin-4 production and B cell expansion. PLoS Pathogens, 17(2), e1009260.
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2

Immunofluorescence Imaging of Lymph Nodes

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The entire collected lymph node was placed in Tissue-Tek optimum cutting temperature compound (Thermo Scientific) and immediately frozen in liquid nitrogen. Serial cryostat sections (10 μm) were collected, air-dried, and fixed in ice-cold 75% acetone/25% ethanol for 5 min. Tissue was rehydrated in PBS for 10 min and blocked using biotin blocking kit (Vector Laboratories, according to manufacturer’s instructions) followed by incubation with 1% v/v in PBS of rat and rabbit serum. Immunofluorescence staining was performed overnight at 4°C with diluted antibodies in blocking buffer. Secondary staining was performed the following morning by incubating with specific antibodies for 1 hour at room temperature. Mounting was done using ProLong anti-fade reagents (Life Technologies) followed by imaging using Leica TCS Sp5 Laser Scanning Microscopy using tile scan feature with an average grid size of 3 × 3 taken with a 20x objective at a resolution of 1024 × 1024. Image postprocessing was done using Fiji is Just ImageJ software (1.47v).
Cortes-Selva D., Ready A., Gibbs L., Rajwa B, & Fairfax K.C. (2019). IL-4 promotes stromal cell expansion and is critical for development of a type-2, but not a type 1 immune response. European journal of immunology, 49(3), 428-442.
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