mRNA was extracted using TRIzol reagent (Invitrogen Corporation) and quantitative reverse transcription-PCR was performed as described.54 (link) Primer sequences are given as Supplementary materials . Total protein extraction and western blot protocol have been previously described.54 (link) Chromatin fractionation was performed as described.57 (link) Primary antibodies were as follows: MYCN (B8.4.B), SC53993, p53 (DO-1) SC-126, CHK1 (G4), SC8408, Tubulin, (TU-02), SC-8035, and β-Actin (I-19) SC-1616 Santa Cruz Biotechnology; phospho-CHK1 (S317), DR1025, Calbiochem (San Diego, CA, USA); RAD50 (13B3/2C6), ab89, Mre11 (12D7) ab214, ZIC1 ab72694, Histone H3, ab1791, phospho-ATM (Ser 1981), ab81292, Rad50, ab499, and Mre11, ab6511, Abcam (Cambridge, UK); NBS1 (1C3) GTX70222, Gene Tex (Irvine, CA, USA); p53 (1C12), #2524, phospho-p53 (Ser 15), #9284, and phospho- histone H2AX (ser 139), #2577, Cell Signaling Technology (Danvers, MA, USA); PARP p85 fragment, #G7341, Promega Corporation; RPA32, A300-244 A, phospho-RPA32 S4/S8, A300-245 A, Bethyl Laboratories (Montgomery, TX, USA); Nbs1 (Y112), NB110-57272, Novus Biologicals; Immunoreactive bands were visualized by enhanced chemoluminescence (Advansta Inc., Menlo Park, CA, USA).
Mre11 12d7 ab214
Manufactured by Abcam
Sourced in United States, United Kingdom
MRE11 (12D7) ab214 is a mouse monoclonal antibody that recognizes the MRE11 protein. MRE11 is a critical component of the MRE11-RAD50-NBS1 (MRN) complex, which is involved in the cellular response to DNA double-strand breaks.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemoluminescence, by Advansta (2 mentions)
β actin 1 19 sc 1616, by Santa Cruz Biotechnology (2 mentions)
Ab81292, by Abcam (1 mentions)
Phospho histone h2a x ser139, by Cell Signaling Technology (1 mentions)
Phospho atm ser 1981, by Abcam (1 mentions)
Ab1791, by Abcam (1 mentions)
Sc 8035, by Santa Cruz Biotechnology (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Histone h3, by Abcam (1 mentions)
Mre11, by Abcam (1 mentions)
Caspase 3 9662, by Cell Signaling Technology (1 mentions)
Phospho p53 ser15, by Cell Signaling Technology (1 mentions)
2 protocols using mre11 12d7 ab214
1
Protein Extraction and Analysis Protocol
2
Protein Extraction and Western Blot
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Total protein extraction and western blot protocols have been previously described56 (link). Immunoreactive bands were visualized by enhanced chemoluminescence (Advansta Inc., Menlo Park, CA, USA). Antibodies were as follows: MRE11 (12D7) ab214 (Abcam, Cambridge, UK); PARP 85 fragment #G7341 (Promega Corporation, Medison, WI, USA); Caspase-3 #9662, phospho-histone H2AX (ser 139) #2577 and phospho-p53 (Ser 15) #9284 (Cell Signaling Technology, Danvers, MA), USA; p53 (DO-1) #SC-126 and β-actin (I–19) #SC-1616 (Santa Cruz Biotechnology, INC, Dallas, TX, USA).
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