Recombinant human TGF-β1 was purchased from PeproTech (Princeton, NJ, USA). SB431542 was purchased from Selleckchem (Houston, TX, USA). RGD peptide was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). TGF-β neutralizing antibody was purchased from R&D Systems (Minneapolis, MN, USA). Anti-TNC antibodies for IHC staining and western blot analysis were purchased from Santa Cruz Biotechnology and Abcam (Cambridge, UK), respectively. Anti-GAPDH antibody was from AbClone (Seoul, Korea). Anti-SMAD2 (SMAD family member 2), anti-phospho-SMAD2, anti-SMAD3, and anti-phospho-SMAD3 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-pro-COL1A1 and anti-MMP-1 antibodies have been described previously [49 (link)]. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, goat anti-rabbit IgG, and rabbit anti-goat IgG antibodies were purchased from KOMA Biotech (Seoul, Korea). Rhodamine Red-X-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated rabbit anti-goat IgG antibodies were purchased from Invitrogen (Carlsbad, CA, USA).
Rhodamine red x conjugated goat anti mouse igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
Rhodamine Red-X-conjugated goat anti-mouse IgG is a secondary antibody reagent used in fluorescence-based applications. It consists of goat-derived polyclonal antibodies that specifically recognize and bind to mouse immunoglobulin G (IgG) molecules. The antibodies are conjugated with the fluorescent dye Rhodamine Red-X, which can be detected using appropriate excitation and emission wavelengths.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg, by Koma Biotech (2 mentions)
Horseradish peroxidase hrp conjugated goat anti mouse igg, by Koma Biotech (2 mentions)
Anti phospho smad3, by Cell Signaling Technology (2 mentions)
Anti smad3, by Cell Signaling Technology (2 mentions)
Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (2 mentions)
Recombinant human tgf β1, by Thermo Fisher Scientific (1 mentions)
Tgf β neutralizing antibody, by R&D Systems (1 mentions)
Anti gapdh antibody, by Abclone (1 mentions)
Rgd peptide, by Santa Cruz Biotechnology (1 mentions)
Sb431542, by Selleck Chemicals (1 mentions)
Alexa fluor 488 conjugated donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated donkey anti goat igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 555 conjugated egf, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase hrp conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti phospho smad2, by Cell Signaling Technology (1 mentions)
Anti smad2, by Cell Signaling Technology (1 mentions)
2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions)
Anti nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Recombinant human tnf α, by Thermo Fisher Scientific (1 mentions)
4 protocols using rhodamine red x conjugated goat anti mouse igg
1
TGF-β1 Signaling Pathway Regulation
2
Imaging of Phosphoinositides in HeLa Cells
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HeLa cells were obtained from the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University (Sendai, Japan). The cells were maintained in DMEM containing 10% fetal bovine serum (FBS) at 37°C under 5% CO2. Mouse monoclonal anti-PtdIns(4)P antibody and mouse monoclonal anti-PtdIns(4,5)P2 antibody were purchased from Echelon (Salt Lake City, UT). Mouse monoclonal anti-EEA1 antibody was purchased from BD Transduction Laboratories (San Diego, CA). Mouse monoclonal anti–transferrin receptor antibody was from Zymed (South San Francisco, CA), and rabbit monoclonal anti-EEA1 antibody was from Cell Signaling Technologies (Beverly, MA). Goat anti-GST antibody and rabbit anti-GST antibody were from Amersham (Piscataway, NJ) and Santa Cruz Biotechnology (Santa Cruz, CA), respectively. Mouse monoclonal anti-PtdIns4KIIα antibody (1C4) has been previously described (Banerji et al., 2010 (link)). Mouse anti-Xpress antibody, Alexa Fluor 488–conjugated Tfn, Alexa Fluor 555–conjugated EGF, Alexa Fluor 488–conjugated donkey anti-mouse immunoglobulin G (IgG), Rhodamine Red X–conjugated goat anti-mouse IgG, Alexa Fluor 555–conjugated goat anti-mouse IgM, Alexa Fluor 488–conjugated donkey anti-goat IgG, and Alexa Fluor 488–conjugated donkey anti-rabbit IgG were purchased from Invitrogen (Carlsbad, CA).
3
Antibody Sourcing for Extracellular Matrix Research
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Anti-pro-collagen α1(1) N-propeptide (pN-Col1 α1) and anti-MMP1 antibodies were obtained as described [52 (link)]. We sourced antibodies from their respective suppliers: anti-SPARC (15274-1-AP; Proteintech, Chicago, IL, USA); anti-GAPDH (ab83108; AbClone, (Seoul, Republic of Korea); anti-SMAD2 (#5339), anti-phospho-SMAD2 (#3108), anti-SMAD3 (#9523), and anti-phospho-SMAD3 (#9520) (all from Cell Signaling Technology, Danvers, MA, USA); anti-collagen type I cleavage site (immunoGlobe Antikörpertechnik GmbH, Himmelstadt, Germany); horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, goat anti-rabbit IgG, Alexa Fluor® 488 goat anti-rabbit IgG (H+L), and rhodamine Red X-conjugated goat anti-mouse IgG (Thermo Fisher Scientific Inc., Waltham, MA, USA) [53 (link)].
4
Investigating Inflammatory Signaling Pathways
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Recombinant human TNF-α was purchased from Peprotech and 2′,7′-dichlorofluorescein diacetate (DCF-DA) was purchased from Sigma–Aldrich. The following antibodies were used: anti-SOD3 for western blotting (ab83108) and anti-SOD3 for immunohistochemistry (IHC; ab171738) from Abcam; anti-glyceraldehyde dehydrogenase (GAPDH; ab83108) from AbClone; anti-phospho-NF-κB inhibitor protein ɑ (IκBɑ), anti-phospho-NF-κB p65, anti-phospho-c-jun N-terminal kinase (JNK), anti-JNK, anti-phospho-p38 mitogen-activated protein kinase (MAPK) (Cell Signaling Technology), anti-NF-κB p65, anti-p38 MAPK, anti-phospho-extracellular signal-regulated kinase (ERK), and anti-ERK2 from Santa Cruz Biotechnology; and anti-collagen type I cleavage site from ImmunoGlobe. Anti-MMP-1 and procollagen α1(I) N-propeptide (pN-ColIα1) antibodies have been previously described [21 (link)]. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were purchased from KOMA Biotech (Seoul, Korea). Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and rhodamine Red-X-conjugated goat anti-mouse IgG were purchased from Thermo Fisher Scientific (Waltham, MA, USA). SPlink HRP Detection Bulk Kit for Mouse and Rabbit Antibodies and Liquid AEC Substrate Kit (20×) for IHC were purchased from GBI labs (Bothell, WA, USA).
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