The samples were analyzed by the reference technique, microbiological culture, followed by PCR confirmation according to the previously described protocol (28 (link)). Briefly, the homogenate was decontaminated with an equal volume of 0.75% (wt/vol: 1/1) hexadecylpyridinium chloride solution in agitation for 30 min. Samples were centrifuged for 30 min at 1,500 × g (28 (link)). The pellets were collected with swabs and cultured in liquid media (MGIT 960, Becton Dickinson, Madrid, Spain) using an automated BD Bacter MGIT System (Becton Dickinson). The culture was considered positive when colonies were confirmed as MTC by real-time PCR (29 (link)).
Bacter mgit system
Manufactured by BD
Sourced in Spain
The BD Bacter MGIT System is a liquid culture-based automated mycobacteria detection and identification system. It is used for the rapid detection and identification of mycobacteria, including Mycobacterium tuberculosis, from clinical specimens.
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2 protocols using bacter mgit system
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Mycobacterium tuberculosis detection protocol
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Mycobacterium tuberculosis detection protocol
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The samples were analyzed by the reference technique, microbiological culture, followed by PCR confirmation according to the previously described protocol (28 (link)). Briefly, the homogenate was decontaminated with an equal volume of 0.75% (wt/vol: 1/1) hexadecylpyridinium chloride solution in agitation for 30 min. Samples were centrifuged for 30 min at 1,500 × g (28 (link)). The pellets were collected with swabs and cultured in liquid media (MGIT 960, Becton Dickinson, Madrid, Spain) using an automated BD Bacter MGIT System (Becton Dickinson). The culture was considered positive when colonies were confirmed as MTC by real-time PCR (29 (link)).
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