For IHC analysis: mouse monoclonal SCF antibody (sc-13126, 1:100 dilution) and mouse monoclonal HIF-1α antibody (sc-13515, 1:100 dilution) were obtained from Santa Cruz Biotechnology.
For western blot analysis: mouse monoclonal HIF-1α antibody (sc-13515, 1:500 dilution), mouse monoclonal β-actin antibody (sc-8432, 1:2500 dilution), and rabbit polyclonal SCF antibody (sc-9132, 1:1000 dilution) were obtained from Santa Cruz Biotechnology. The secondary antibodies preparation was either anti-rabbit (1:5000) or anti-mouse (1:5000).
For cell functional experiments: rabbit polyclonal neutralizing SCF antibody (ab9716, 0.01 μg/mL) was obtained from Abcam.
Gao C., Li S., Zhao T., Chen J., Ren H., Zhang H., Wang X., Lang M., Liu J., Gao S., Zhao X., Sheng J., Yuan Z, & Hao J. (2015). SCF, Regulated by HIF-1α, Promotes Pancreatic Ductal Adenocarcinoma Cell Progression. PLoS ONE, 10(3), e0121338.
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