Bestatin, Chymostatin, E-64, Leupeptin, Pepstatin, Phosphoramidon, Pefabloc SC, EDTA, Aprotinin, Antipain were purchased from Roche as part of a protease inhibitors set and used at the following concentrations: 74 μM Antipain; 130 μM Bestatin; 100 μM Chymostatin; 28 μM E-64; 1 μM Leupeptin; 1 μM Pepstatin; 600 nM Phosphoramidon; 4 mM Pefabloc SC; 1.3 mM EDTA; 0.3 mM Aprotinin. The additional compounds ATP (Boehringer), Bacitracin (Applichem), insulin from bovine pancreas (Sigma), PMSF (Sigma), 6bK [45 (link)] (Phoenix Pharmaceuticals and Tocris), TPEN (Calbiochem), and NEM (Roche) were purchased from companies indicated.
Hahn F., Schmalen A., Setz C., Friedrich M., Schlößer S., Kölle J., Spranger R., Rauch P., Fraedrich K., Reif T., Karius-Fischer J., Balasubramanyam A., Henklein P., Fossen T, & Schubert U. (2017). Proteolysis of mature HIV-1 p6 Gag protein by the insulin-degrading enzyme (IDE) regulates virus replication in an Env-dependent manner. PLoS ONE, 12(4), e0174254.
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