Human vegf standard elisa development kit

PLGA with an inherent viscosity (i.v.) of 0.57 dLg ⁻¹ (50:50, PLGA DL LOW IV, lot # A11-071, lauryl ester end group, 51 kDa) was purchased from Lakeshore Biomaterials (Birmingham, Alabama). High molecular weight (~ 500 kDa) dextran sulfate (HDS), low molecular weight (~15.5 kDa) dextran sulfate (LDS), chondroitin sulfate (~ 63 kDa, shark cartilage) (CS), porcine heparin (~18 kDa) (HP) sodium salts were purchased from Sigma-Aldrich. Chitosan (~ 61 kDa) was also from Sigma-Aldrich. Sodium hyaluronate (HA) powders and VEGF165 were obtained as a gift from Lifecore Biomedical (MN, USA) and Genentech, respectively. Lysozyme (chicken egg white) and magnesium carbonate were purchased from Sigma-Aldrich. FgF-20 was obtained from the National Cancer Institute-Biometric Research Branch (NCI-BRB) preclinical repository. Human VEGF standard ELISA development kit was purchased from Peprotech (NJ, USA). Cyanine-5 labelled lysozyme was bought from Nanocs (NY, USA). All other reagents, common solvents, and supplies were obtained from Sigma-Aldrich, unless otherwise specified.