Ccr7 clone 3d12

Manufactured by BD

Sourced in Denmark

CCR7 (clone 3D12) is a lab equipment product that serves as a core component in various research applications. The product functions as a specific antibody that binds to the CCR7 chemokine receptor, a key regulator of lymphocyte trafficking and homing. This antibody can be utilized in flow cytometry, immunohistochemistry, and other immunological techniques to identify and study CCR7-expressing cells. The detailed specifications and intended use of this product are not available within the scope of this request.

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Lab products found in correlation

Cd86 clone it2, by BD (1 mentions) Cd83 clone hb15e, by BD (1 mentions) Cd40 clone 5c3, by BD (1 mentions) Ccr6 clone 11a9, by BD (1 mentions) Hla dr clone g46 6, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Hla dr, by BD (1 mentions) Ebio64dec17, by Thermo Fisher Scientific (1 mentions) 8 color canto 2 flow cytometer, by BD (1 mentions) Cd45ra clone hi100, by BD (1 mentions) Cd4 clone rpa t4, by BioLegend (1 mentions) Cd3 clone sk7, by BioLegend (1 mentions)

Close spelling variants of this product

CCR7-PE-Cy7 (clone 3D12, BB700 anti-CCR7 (clone 3D12, Anti-human CD197(CCR7)-PE (1/50) clone 3D12 Anti-CCR7–PE-Cy7 (clone 3D12) CCR7 (3D12;

3 protocols using ccr7 clone 3d12

VLP Binding and DC Activation Assay

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Titrated Alexa Fluor 647 labelled-VLP stocks were used to stain cDCs, LNCaP and HEK 293 TT cells at the indicated concentration. VLP attachment was detected by flow cytometry gated on DAPI negative cells. To assess DC activation, cells were incubated for 24 hours with 10³ VLPS/cell, 10³ BKPyV particles/cell or with 100ng/mL LPS and 1μg/mL R848 (Invivogen, San Diego, CA). Antibodies to CD40 (clone 5C3; BD Biosciences), CD80 (clone L307, BD Biosciences), CD83 (clone HB15e, BD Biosciences), CD86 (clone IT2.2, BD Biosciences), CCR6 (clone 11A9, BD Biosciences), CCR7 (clone 3D12, BD Biosciences) and HLA-DR (clone G46-6, BD Biosciences) were used to monitor DC maturation. Whole blood staining was performed on 500μl blood samples from healthy donors with or without Fc fragment receptor blockers (Miltenyi Biotec). Whole blood staining was done with Alexa Fluor 647 labelled-VLPS (2.5μg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15; Beckman Coulter), HLA-DR (Clone L243; BD Biosciences), CD123 (Clone 9F5; BD Biosciences) and Lineage (Lin 1; BD Biosciences). FACS analyses were mainly performed on a LSR II flow cytometer (BD Biosciences).

Sikorski M., Coulon F., Peltier C., Braudeau C., Garcia A., Giraud M., Renaudin K., Kandel-Aznar C., Nedellec S., Hulin P., Branchereau J., Véziers J., Gaboriaud P., Touzé A., Burlaud-Gaillard J., Josien R., McIlroy D., Bressollette-Bodin C, & Halary F. (2021). Non-permissive human conventional CD1c+ dendritic cells enable trans-infection of human primary renal tubular epithelial cells and protect BK polyomavirus from neutralization. PLoS Pathogens, 17(2), e1009042.

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Phenotyping of T cell subsets

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Phenotyping of cell surface markers on frozen PB cells was performed using antibodies to CD4 (clone OKT4), CD25 (clone 2A3), CD127 (clone HIL-7R-M21), CD146 (clone P1H12), CCR5 (clone 2D7), CD45RA (clone HI100), and CCR7 (clone 3D12) (all from BD Biosciences). Intracellular staining of transcription factors (RORC, clone Q21-559, and TBET, clone 4B10) and cytokines (IL-17, clone eBio64DEC17, and IFN-γ, clone 4S.B3) was performed after fixation and permeabilization according to the manufacturer's recommendations using the buffer set (eBioscience). Tcons were defined as CD4⁺CD25^loCD127⁺, and Tregs were defined as CD4⁺CD25⁺CD127⁻FOXP3⁺. Flow cytometric analysis was performed using an 8-color Canto II flow cytometer (BD Biosciences) and FlowJo software (Tree Star Inc.). Absolute counts of T cell subsets were calculated by multiplying the frequency of T cells among lymphocytes by the ALC obtained using the automated method.

Li W., Liu L., Gomez A., Zhang J., Ramadan A., Zhang Q., Choi S.W., Zhang P., Greenson J.K., Liu C., Jiang D., Virts E., Kelich S.L., Chu H.W., Flynn R., Blazar B.R., Hanenberg H., Hanash S, & Paczesny S. (2016). Proteomics analysis reveals a Th17-prone cell population in presymptomatic graft-versus-host disease. JCI Insight, 1(6), e86660.

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Multiparameter Flow Cytometry Analysis

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Aliquots of transduced T cells were analyzed by 7-color flow cytometry using a panel of surface molecule specific antibodies, peptide tetramer, and dextramer: CD3 (clone SK7, Biolegend), CD4 (clone RPA-T4; Biolegend), CD8 (clone 3B5; Biolegend), anti-IgG (polyclonal Goat anti-human; Jackson ImmunoResearch), CD45RA (clone MEM-56; Thermo Fisher), and CCR7 (clone 3D12; BD Biosciences); HLA-A*0201 PR1 tetramer (Baylor tetramer core facility), HLA-A*0201 CMV pp65 dextramer and HLA-A*0201 PR1 dextramer (Immudex, Denmark).

Ma Q., Garber H.R., Lu S., He H., Tallis E., Ding X., Sergeeva A., Wood M.S., Dotti G., Salvado B., Ruisaard K., Clise-Dwyer K., John L.S., Rezvani K., Alatrash G., Shpall E.J, & Molldrem J.J. (2016). A novel TCR-like CAR with specificity for PR1/HLA-A2 effectively targets myeloid leukemia in vitro when expressed in human adult peripheral blood and cord blood T cells. Cytotherapy, 18(8), 985-994.

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