AAV2-hSyn-hChR2(H134R)-mCherry (titer: 2.9e12), AAV2-hSyn-mCherry (titer: 4.7e12), and AAV2-hSyn-eNpHR3.0-mCherry (1.5e12) were obtained from the University of North Carolina Vector Core with available stock constructs from the laboratory of K. Deisseroth at Stanford University.
C-Fos antibody (1:5000, Cell Signaling Technology Cat# 4384, RRID:AB_2106617) was a rabbit polyclonal antibody raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human c-Fos protein. TH antibody (1:250, Millipore Cat# AB152, RRID:AB_390204) was a rabbit polyclonal antibody raised against denatured tyrosine hydroxylase from rat pheochro-mocytoma. NeuN antibody (1:1000, EMD Millipore Cat# MAB377, RRID:AB_2298772) was a mouse monoclonal antibody, clone A60, selected from immunoglobulins formed against purified cell nuclei from mouse brain. Secondary antibodies used were purchased from Jackson Immuno Research (Westgrove, PA): Cy5 Goat anti-rabbit IgG (1:500, Cat# 111-175-144, RRID:AB_2338013), Alexafluor 594 Donkey anti-rabbit IgG (1:500, Cat# 711-585-152, RRID:AB_2340621), Cy5 Goat anti-mouse IgG (1:500, Cat# 115-175-146, RRID:AB_2338713), and FITC streptavidin (1:200, Cat# 016-010-084, RRID:AB_2337236).
C-Fos antibody (1:5000, Cell Signaling Technology Cat# 4384, RRID:AB_2106617) was a rabbit polyclonal antibody raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human c-Fos protein. TH antibody (1:250, Millipore Cat# AB152, RRID:AB_390204) was a rabbit polyclonal antibody raised against denatured tyrosine hydroxylase from rat pheochro-mocytoma. NeuN antibody (1:1000, EMD Millipore Cat# MAB377, RRID:AB_2298772) was a mouse monoclonal antibody, clone A60, selected from immunoglobulins formed against purified cell nuclei from mouse brain. Secondary antibodies used were purchased from Jackson Immuno Research (Westgrove, PA): Cy5 Goat anti-rabbit IgG (1:500, Cat# 111-175-144, RRID:AB_2338013), Alexafluor 594 Donkey anti-rabbit IgG (1:500, Cat# 711-585-152, RRID:AB_2340621), Cy5 Goat anti-mouse IgG (1:500, Cat# 115-175-146, RRID:AB_2338713), and FITC streptavidin (1:200, Cat# 016-010-084, RRID:AB_2337236).