Freshly prepared cell suspensions were performed immediately according to the manufacturer’s protocol of 10 X Chromium 3’ v3 kit (10x Genomics, Pleasanton, CA). Library was prepared and sequencing was performed on a NovaSeq 6000 platform (Illumina, Inc., San Diego, CA) in GENERGY BIO (Shanghai, China).
Chromium 3 v3 kit
Manufactured by 10x Genomics
The 10X Chromium 3' v3 kit is a laboratory equipment designed to perform single-cell gene expression analysis. The kit enables the capture, barcoding, and sequencing of individual cells, allowing for the study of gene expression profiles at the single-cell level.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using chromium 3 v3 kit
1
Single-Cell RNA-Seq Library Preparation
2
Single-Cell RNA-Seq of Intestinal Cells
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Intestinal single cells were resuspended in PBS supplemented with 0.04% BSA. Single-cell transcriptomic amplification and library preparation were performed using 10 X Chromium 3' v3 kit (10x Genomics) according to manufacturer's instructions. Sequencing was performed on a NovaSeq 6000 platform in Shanghai Institute of Immunology.
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