Elastin orcein

Recombinant human tropoelastin was kindly provided by Anthony S. Weiss from the University of Sydney, Australia. Elastin-orcein, the chemical cross-linker bis(sulfosuccinimidyl) suberate (BS3 (link)), bovine serum albumin (BSA), and bovine neck ligament elastin isoform 1 (SwissProt accession number P04985–1, hereafter referred to as bovine elastin) were obtained from Sigma (USA). FAGLA was synthesized by ChinaPeptides Co., Ltd. (China). Recombinant pseudolysin was expressed in Escherichia coli and purified as previously described29 (link).

Bovine tendon collagen was purchased from Worthington (Lakewood, NJ, USA), and bovine bone collagen from Kinry Biotech Co., Ltd. (Jinan, China). Casein (from bovine milk), gelatin (from cold water fish skin), elastin-orcein, EGTA, o-P, aprotinin, and cytochrome C were purchased from Sigma (St. Louis, MO, USA). Alcalase was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). PMSF was purchased from BBI (Shanghai, China). EDTA was purchased from HUSHI (Shanghai, China). Bacitracin was purchased from Aladdin (Shanghai, China). Tripeptide GGG and tetrapeptide GGYR were synthesized by Qiangyao Biotechnology Co., Ltd. (Shanghai, China). DPPH• was purchased from Tokyo Chemical Industry (Tokyo, Japan). HA was purchased from Shandong Freda Bioeng Co., Ltd. (Jinan, China). Other chemicals were of analytical grade and commercially available.