B6 mESCs used in this study were established and maintained in 2i/LIF medium as previously described (Pryzhkova and Jordan, 2016 (link)). 129S6/SvEvTac (129S6) mESCs (Taconic Biosciences) were obtained from JHU Transgenic Mouse Core. mESCs from TIR1 transgenic animals were established and maintained in 2i/LIF medium as described in (Pryzhkova and Jordan, 2016 (link)) with exception that mESCs were established from pooled embryos. Briefly, mESC culture medium included 1:1 mixture of DMEM/F12 (Gibco) and Neurobasal medium (Gibco) with 1% N2 supplement (Gibco) and 2% B27 supplement (Gibco), 1 mM L-Glutamine (Gibco), 1% MEM NEAA (Gibco), 50 μM β-mercaptoethanol (Gibco), 50 μg/ml BSA (Sigma), 10 ng/ml human LIF (PeproTech), 1 μM MEK inhibitor PD 0325901 (Cayman) and 3 μM GSK-3 inhibitor CHIR 99021 (Cayman). Cells were grown under feeder-free conditions on 0.2% gelatin (Sigma) and passaged every 3 days with TrypLE Express (Gibco). Essential reagent information is provided in Table S3 .
129s6 svevtac 129s6 mescs
Manufactured by Taconic Biosciences
The 129S6/SvEvTac (129S6) mESCs are a mouse embryonic stem cell line derived from the 129S6/SvEvTac mouse strain. These cells maintain pluripotency and can differentiate into various cell types. The 129S6 mESCs provide a standardized model for a range of research applications.
Automatically generated - may contain errors
Lab products found in correlation
B27 supplement, by Thermo Fisher Scientific (2 mentions)
Human lif, by Thermo Fisher Scientific (2 mentions)
Gsk 3 inhibitor chir 99021, by Cayman Chemical (2 mentions)
Mek inhibitor pd0325901, by Cayman Chemical (2 mentions)
N2 supplement, by Thermo Fisher Scientific (2 mentions)
Mem neaa, by Thermo Fisher Scientific (2 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Neurobasal medium, by Thermo Fisher Scientific (2 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
2 protocols using 129s6 svevtac 129s6 mescs
1
Maintenance of Mouse Embryonic Stem Cells
2
Maintenance of Mouse Embryonic Stem Cells
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B6 mESCs used in this study were established and maintained in 2i/LIF medium as previously described (Pryzhkova and Jordan, 2016 (link)). 129S6/SvEvTac (129S6) mESCs (Taconic Biosciences) were obtained from JHU Transgenic Mouse Core. mESCs from TIR1 transgenic animals were established and maintained in 2i/LIF medium as described in (Pryzhkova and Jordan, 2016 (link)) with exception that mESCs were established from pooled embryos. Briefly, mESC culture medium included 1:1 mixture of DMEM/F12 (Gibco) and Neurobasal medium (Gibco) with 1% N2 supplement (Gibco) and 2% B27 supplement (Gibco), 1 mM L-Glutamine (Gibco), 1% MEM NEAA (Gibco), 50 μM β-mercaptoethanol (Gibco), 50 μg/ml BSA (Sigma), 10 ng/ml human LIF (PeproTech), 1 μM MEK inhibitor PD 0325901 (Cayman) and 3 μM GSK-3 inhibitor CHIR 99021 (Cayman). Cells were grown under feeder-free conditions on 0.2% gelatin (Sigma) and passaged every 3 days with TrypLE Express (Gibco). Essential reagent information is provided in Table S3 .
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