Ionomycin, ω-conotoxin GVIA, ω-agatoxin IVA, and SNX-482 were purchased from Alomone labs (catalog numbers I-700, C-300, STA-500, and RTS-500 respectively). The toxins were applied for 3 min in Tyrode’s solution at concentrations of 1 µM, 400 nM, and 500 nM for ω-conotoxin GVIA, ω-agatoxin IVA, and SNX-482, respectively (Ariel et al., 2012 (link); Ermolyuk et al., 2013 (link); Hoppa et al., 2012 (link)). Baclofen was purchased from Sigma-Aldrich (catalog number B 5399) and continuously perfused at 10 µM beginning 5 min before AP stimulation (Laviv et al., 2011 (link)).
Sta 500
Manufactured by Alomone
The STA-500 is a multi-channel potentiostat/galvanostat that can be used for electrochemical analysis and testing. It provides accurate and reliable measurements for a variety of electrochemical techniques.
Automatically generated - may contain errors
2 protocols using sta 500
1
Calcium Channel Modulation in Neurons
2
Vesicle Imaging with Toxin Inhibition
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FM 1–43 was loaded into synaptic vesicles as described above; 2 min after loading, the chamber was perfused with ACSF for 10 min. The perfusion solution was then switched to ACSF containing 100 nM ω-conotoxin GVIA (C-300, Alomone Labs) or 200 nM ω-agatoxin GVIA (STA-500, Alomone Labs); 2 min later, FM 1–43 imaging was performed as described above. Data was analyzed as described above.
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