Pi p1304mp

Manufactured by Thermo Fisher Scientific

Sourced in United States

The PI P1304MP is a precision linear actuator designed for a variety of industrial and laboratory applications. It features a high-resolution stepper motor and a precision lead screw mechanism to provide smooth and accurate linear motion control. The core function of this product is to enable precise positioning and movement of objects or samples within a laboratory or industrial setting.

Automatically generated - may contain errors

Lab products found in correlation

Diisopropylcarbodiimide dic, by Merck Group (1 mentions) Ethyl cyanohydroxyiminoacetate oxyma, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) Rhodamine phalloidin, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Cck 8, by Beyotime (1 mentions)

2 protocols using pi p1304mp

Curcumin-Based Antimicrobial and Cytotoxicity Assays

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Curcumin ≥65% pure
(Cur), fmoc-Arg(Pbf)-OH, diisopropylcarbodiimide (DIC), piperidine,
ethylcyano(hydroxyimino)acetate (Oxyma), N,N-dimethylformamide anhydrous (DMF), dichloromethane, trifluoroacetic
acid (TFA), triisopropylsilane (TIS), and dimethyl sulfoxide (DMSO)
were purchased from Sigma-Aldrich. PI P1304MP and LIVE/DEAD Baclight assay kit (L7007) were purchased from Invitrogen
(Carlsbad, CA, USA). Methylthiazolyldiphenyl-tetrazolium bromide (MTT)
reagent (M2128) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
Bacterial cells E. coli (ATCC 25922)
and S. aureus (ATCC 25923) were procured
from Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh,
India. NIH3T3 mouse fibroblast cells were obtained from National Centre
for Cell Science (NCCS) Pune, India. Dulbecco’s modified Eagle’s
medium, trypsin, was obtained from Invitrogen. Mueller Hinton (MH)
agar and broth were obtained from HiMedia (Mumbai, India).

Ratrey P., Dalvi S.V, & Mishra A. (2020). Enhancing Aqueous Solubility and Antibacterial Activity of Curcumin by Complexing with Cell-Penetrating Octaarginine. ACS Omega, 5(30), 19004-19013.

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Proliferation Assessment of BMSC Hydrogels

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Live/Dead staining and CCK-8 were applied to evaluate the proliferation of BMSCs cultured in hydrogels. For CCK-8 test, every hydrogel piece was incubated in 200 μL medium containing 10% CCK-8 (C0037, Beyotime, China) for 3 h, and OD value was recorded at 450 nm. As for the Live/Dead cell staining, the samples were soaked in PBS solution, which contained fluorescein diacetate (FDA, F1303, Invitrogen) (1‰, v/v) and propidium iodide (PI, P1304MP, Invitrogen) (1‰, v/v), and observed by CLSM. The cytoskeleton of the BMSCs in hydrogels was exhibited using rhodamine-phalloidin (1 h, 94072, Sigma-Aldrich) and DAPI (1 min, 28718-90-3, Sigma-Aldrich), which was imaged by CLSM.

Zhao C., Li X., Han X., Li Z., Bian S., Zeng W., Ding M., Liang J., Jiang Q., Zhou Z., Fan Y., Zhang X, & Sun Y. (2024). Molecular co-assembled strategy tuning protein conformation for cartilage regeneration. Nature Communications, 15, 1488.

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