E coli single use jm109 competent cells

Manufactured by Promega

Sourced in Germany

E. coli Single-Use JM109 Competent Cells are a pre-made preparation of chemically competent Escherichia coli JM109 bacterial cells. These cells are optimized for efficient transformation of plasmid DNA.

Automatically generated - may contain errors

Lab products found in correlation

Clonejet pcr cloning kit, by Thermo Fisher Scientific (2 mentions) Nucleospin gel and pcr clean up, by Macherey-Nagel (2 mentions)

Close spelling variants of this product

E. coli JM109 competent cells E. coli JM109 cells JM109 competent cells E. coli JM109 JM109 cells JM109

2 protocols using e coli single use jm109 competent cells

S7-specific primers design and cloning

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In order to design S₇-specific primers, a fragment of 900 bp obtained by using the primers PruC2/PruC4R was isolated using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Cloning was performed using CloneJET PCR Cloning Kit (Thermo Scientific, St Leon-Rot, Germany) and by electroporation in E. coli Single-Use JM109 Competent Cells (Promega Biotech Ibérica SL, Madrid, Spain). The search for similarities in the sequences of the NCBI database was performed with BLAST (http://www.ncbi.nlm.nih.gov/BLAST, version 2.2.10).

Herrera S., Rodrigo J., Hormaza J.I, & Lora J. (2018). Identification of Self-Incompatibility Alleles by Specific PCR Analysis and S-RNase Sequencing in Apricot. International Journal of Molecular Sciences, 19(11), 3612.

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Cloning and Sequencing of PCR Fragments

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Two PCR fragments of 420 and 430 bp obtained by the automatic sequencer were isolated using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Cloning was performed using CloneJET PCR Cloning Kit (Thermo) and by electroporation in E. coli Single-Use JM109 Competent Cells (Promega). The search for similarities in the sequences of the NCBI database was performed with BLAST (http://www.ncbi.nlm.nih.gov/BLAST, version 2.2.10). The 420 bp fragment resulted in a fragment of 414 bp after one sequencing reaction whereas the initial 430 bp fragment resulted in a fragment of 421 bp after two sequencing reactions.

Herrera S., Lora J., Hormaza J.I., Herrero M, & Rodrigo J. (2018). Optimizing Production in the New Generation of Apricot Cultivars: Self-incompatibility, S-RNase Allele Identification, and Incompatibility Group Assignment. Frontiers in Plant Science, 9, 527.

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