Recombinant CCN1 and CCN1-D125A mutant proteins were produced using a baculovirus expression system in Sf9 insect cells and purified by ion exchange or immuno-affinity chromatography73 (link),74 (link). Sf9 cells (obtained from American Type Culture Collection, CRL-1711™) were routinely tested for mycoplasma contamination using LookOut® Mycoplasma PCR detection kit (Sigma). Purified CCN1 proteins were regularly examined for purity and contamination by SDS-gel electrophoresis and Limulus amoebocyte lysate method (for endotoxin) and also tested for specific activities in comparison with commercially available CCN1 protein (CCN1-Fc chimera protein; R&D systems, 4055-CR; Supplementary Fig. S5B ). Integrin αvβ3/αvβ5 inhibitor (SB273005; S7540), NF-κB inhibitor (Bay11-7082; S2913), γ-secretase inhibitor (DAPT; S2215), YAP-TEAD association inhibitors (Super-TDU; S8554, Verterporfin; S1786), FAK inhibitors (GSK2256098; S8523), and GSK3β inhibitor (CHIR99021; CT99021) were purchased from Selleckchem. Src inhibitor (Dasatinib; SML2589) was from Sigma–Aldrich. Recombinant Jag1 protein (599-JG) and Dkk1 protein (5897-DK) were from R&D systems. A neutralizing anti-Jag1 antibody (HMJ1-29) was from Invitrogen (16-3391-85) and an anti-Dkk1 antibody was from R&D systems (AF1096). 4′,6-diamidino-2-phenylindole (DAPI) solution (#62248) was from Thermo Fisher scientific.
Super tdu s8554
Manufactured by Selleck Chemicals
Sourced in China
The Super-TDU; S8554 is a thermal desorption unit designed for analytical applications. It is a self-contained instrument that facilitates the concentration and transfer of volatile or semi-volatile organic compounds from a sample prior to analysis by gas chromatography or other analytical techniques. The Super-TDU; S8554 allows for precise control of temperature, flow, and timing parameters to maximize analytical performance.
Automatically generated - may contain errors
Lab products found in correlation
Chir 99021 ct99021, by Selleck Chemicals (1 mentions)
S2215, by Selleck Chemicals (1 mentions)
4 6 diamidino 2 phenylindole dapi solution, by Thermo Fisher Scientific (1 mentions)
S2913, by Selleck Chemicals (1 mentions)
Lookout mycoplasma pcr detection kit, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Smmc 7721, by National Collection of Authenticated Cell Cultures (1 mentions)
Mhcc 97h, by National Collection of Authenticated Cell Cultures (1 mentions)
Mhcc 97l, by National Collection of Authenticated Cell Cultures (1 mentions)
Sk hep1, by National Collection of Authenticated Cell Cultures (1 mentions)
Snu 423, by National Collection of Authenticated Cell Cultures (1 mentions)
Snu 475, by National Collection of Authenticated Cell Cultures (1 mentions)
2 protocols using super tdu s8554
1
Recombinant CCN1 Protein Production
2
Liver Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Liver cancer cell lines of human, including HepG2, Huh-7, LM3, MHCC-97H, MHCC-97L, SK-Hep1, SMMC-7721, SNU-423 and SNU-475 were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Liver cancer cells were cultured in a speci c culture medium according to ATCC instructions, supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% (v/v) penicillin (100 U/ml) and astreptomycin (0.1 mg/ml). All of above cells were incubated in a humidi ed incubator under 5% CO 2 at 37 °C. Vertepor n (VP, S1786) and Super-TDU (S8554) were purchased from Selleck (Shanghai, China). Huh-7 cells were treated with 50 nM Vertepor n or 50 nM Super-TDU (1-31), respectively.
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