Neurotrophin 3

Manufactured by Merck Group

Sourced in United States

Neurotrophin-3 is a lab equipment product that serves as a neurotrophic factor. It plays a role in the survival, growth, and differentiation of certain neurons.

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Lab products found in correlation

Brain derived neurotrophic factor (bdnf), by Merck Group (3 mentions) Poly l ornithine, by Merck Group (2 mentions) Organotypic membranes, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Gap19, by Bio-Techne (1 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) Anti tyrosine hydroxylase, by Merck Group (1 mentions) Ionomycin, by Merck Group (1 mentions) Bapta am, by Thermo Fisher Scientific (1 mentions) Ly294002, by Merck Group (1 mentions) U73122, by Merck Group (1 mentions) Bez235, by Selleck Chemicals (1 mentions) Pd0325901, by Selleck Chemicals (1 mentions) Tubb3, by Fortrea (1 mentions) Laminin, by Merck Group (1 mentions)

Close spelling variants of this product

Neurotrophin 3 (NT3; (2 citations)

4 protocols using neurotrophin 3

Cochlear Explant Synaptogenesis Assay with Stem Cells

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The cochlear explants were dissected from early postnatal rats using methods previously described [16 (link)]. For synaptogenesis assays, the hair cells were dissected from the peripheral processes of ANs, thereby denervating the cochlear explant, using techniques previously described [3 (link), 17 (link)]. The cochlear explants and denervated cochlear explants (hair cells only) were grown on 0.4 μm organotypic membranes (Millipore) in coculture media containing NBM supplemented with brain-derived neurotrophic factor and neurotrophin-3 (each added to give a final concentration of 10 ng/mL; Chemicon).
Whole cochlear explants were cocultured with either 21-day-old hiPSC or hESC neurospheres (Figure 1). Denervated cochlear explants were cocultured with either 21 DIV or 28 DIV hiPSC or hESC neurospheres. The cocultures were incubated at 37°C, 10% CO₂ for 1 DIV (explant only control), or 10 DIV (stem cell + explant/denervated explant cocultures). The coculture media were replenished every 2 DIV (100 μL/membrane).

Gunewardene N., Crombie D., Dottori M, & Nayagam B.A. (2016). Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro. Stem Cells International, 2016, 1781202.

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Isolation and Coculture of Cochlear Cells

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Physical ablation was performed to dissect cochlea into the inner H.C. (IHC), outer H.C. (OHC), and surrounding supporting cells from the newborn mice, transferred to laminin (25 mg/mL) and poly-L-ornithine (0.01%, Sigma-Aldrich) coated cover glass and maintained in DMEM/F12 medium supplemented with N2 and B27 overnight at 37 8C. 10% fetal bovine serum (Sigma-Aldrich), 50 ng/mL neurotrophin-3 (Chemicon, Temecula, CA, U.S.), and 10 ng/ mL brain-derived neurotrophic factor (Chemicon) were added during the first day of culture, and the culture medium was refreshed every 2-3 days.
Physiology International 108 (2021) 1, 43-53 Spiral ganglion neuron isolation 0.25% trypsin (15 min, 37 8C) was utilized to isolate spiral ganglion neurons (SGNs) from the modiolus. DMEM/F12 with 10% FBS was added to neutralize the trypsin, and the neurons were harvested by centrifugation. The cell pellet formed was resuspended in DMEM/F12 supplemented with N2 and B27 to make a single-cell suspension. The collected neurons were cocultured with deafferented Corti. Gap19 (50 mM, Tocris Bioscience, Sussex, UK) was added to the culture medium for six days to inhibit Cx43 hemichannels.

Wang J, & Song Q. (2021). Inhibition of connexin 43 induces hearing loss in postnatal mice. Physiology international.

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Antibody Validation for Cellular Analysis

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Antibodies were previously validated for the applications used. The dilutions and applications were as follows: Coronin-1a (Abcam, ab53395, 1:400 for immunohistochemistry); Tubb3 (Covance, MMS-435P- 250, 1:400 for immunohistochemistry); Rhodamine Phalloidin (Life Technologies, R-415 1:400 for immunohistochemistry); Phospho-p44/42 MAPK (Erk1/2) Mouse mAb (Cell signaling, #9106, 1:1000 for western blot); pan-p44/42 MAPK(Erk1/2) antibody(Cell signaling, #9102s, 1:1000 for western blot); Anti-Tyrosine Hydroxylase (Millipore, AB1542 1:130 for immunohistochemistry); horseradish peroxidase-conjugated donkey anti-sheep IgG (Fisher/Jackson Immuno Research, NC9754415 1:250); 3,3′-Diaminobenzidine tetrahydrochloride tablet (Sigma, D5905-50TAB, 1 tablet: 20ml for staining); Neurotrophin 3 (Millipore, GF031); U-73122 (Sigma, U6756); GSK3β Inhibitor XIX (Millipore, 361565); Ionomycin (Sigma, I9657); BAPTA-AM (Invitrogen, B-1205); LY294002 (Sigma, L9908); BEZ235 (Selleckchem, S1009); PD0325901 (Selleckchem, S1036).

Keeler A.B., Suo D., Park J, & Deppmann C.D. (2017). Delineating neurotrophin-3 dependent signaling pathways underlying sympathetic axon growth along intermediate targets. Molecular and cellular neurosciences, 82, 66-75.

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Neurosphere Differentiation Protocol

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The six-well plates were coated with 20 mg/ml poly-L-ornithine (product no. P4957; Sigma-Aldrich) for at least 2 h in the incubator. The poly-L-ornithine solution was then removed, and the plate was rinsed once with water. Laminin (5 mg/ml) (product no. L2020; Sigma-Aldrich) was added to the plate, which then remained in the incubator for 1 h. Phosphate-buffered saline (PBS) was then used to rinse the plate once immediately before use. The neurospheres were mechanically dissociated and digested with 0.25% trypsin for 10 min. Single cells were then plated on coated six-well plates and mixed with glial cell culture medium (kindly provided by Dr. Zhi-Zhao Ma) with DMEM and L-glutamine at a ratio of 1:1, plus 1% FBS, 100 nM all-trans-retinoic acid (product no. R2500; Sigma-Aldrich), 20 ng/ml brain-derived neurotrophic factor (product no. 203702; Millipore, Billerica, MA, USA), and 20 ng/ml neurotrophin-3 (product no. GF308; Millipore).

Zhong Z.Y., Shi B.J., Zhou H, & Wang W.B. (2018). CD133 expression and MYCN amplification induce chemoresistance and reduce average survival time in pediatric neuroblastoma. The Journal of International Medical Research, 46(3), 1209-1220.

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