U 290 uv vis spectrometer

NMR spectra were recorded on a spectrometer at 400 MHz for 1 H and 100 MHz for 13 C NMR. Chemical shifts were given in ppm (δ scale), coupling constant (J) in Hz. was used as deuterated solvent in the preparation of samples for NMR analysis. Column chromatography was performed on silica Merck) . TLC was carried out on analytical alumina plates (60 F 254 ). Hitachi U-290 UV-Vis spectrometer was used for UV measurements. HRMS analyses were recorded on Agilent 6210 LC-TOF/MS spectrometer.
HPLC Perkin Elmer Series 200 was used for isolation of secondary metabolites. Ammonium thiocyanate and BHT were purchased from E. Merck (Darmstadt, Germany). Ferrous chloride, α-tocopherol, polyoxyethylene sorbitan monolaurate (Tween-20), radical 1,1diphenyl-2-picryl-hydrazyl (DPPH • ), 3-(2-pyridyl)-5,6-bis(4-phenyl-sulphonic acid)-1,2,4triazine (Ferrozine), nicotinamide adenine dinucleotide (NADH), BHA, and trichloracetic acid (TCA), formic acid purchased from Sigma-Aldrich (Darmstadt, Germany).