Diallyl trisulfide was purchased from LKT Laboratories (St. Paul, MN, USA). Streptozotocin (STZ), 4’, 6-diamino-2-phenylindole (DAPI) and Compound C (Com.C) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium pentobarbital, Evans blue (EB) and triphenyltetrazolium chloride (TTC) were purchased from Solarbio Life Sciences (Beijing, China). 2-methoxyestradiol (2ME) was purchased from Selleck Chemicals (Houston, TX, USA). LY294002 and BCA Protein Assay Kit were purchased from Beyotime Biotechnology (Shanghai, China). Terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay kit was purchased from Roche (Mannheim, Germany). H9c2 cardiomyoblasts were obtained from Tiancheng biotechnology (Shanghai, China). The primary antibodies against p-AMPK (Thr172), AMPK, cleaved caspase-3, Bcl-2 and Bax were obtained from Cell Signaling Technology (Boston, MA, USA). The primary antibodies against p-ACC (Ser79), ACC, p-AKT (Thr308), AKT, p-GSK3β (Ser9), GSK3β, HIF-1α and β-actin were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The secondary antibodies were purchased from the Zhongshan Company (Beijing, China).
Terminal deoxynucleotidyltransferase mediated dutp nick end labeling tunel assay kit
Manufactured by Roche
Sourced in Germany
The Terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay kit is a laboratory tool used to detect and quantify apoptosis, or programmed cell death, in a sample. The kit utilizes an enzyme, terminal deoxynucleotidyl transferase, to label the fragmented DNA in apoptotic cells with a detectable marker, allowing for their identification and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Bcl2 associated x (bax), by Cell Signaling Technology (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
Evans blue, by Solarbio (1 mentions)
2 methoxyestradiol 2me, by Selleck Chemicals (1 mentions)
Triphenyl tetrazolium chloride (ttc), by Solarbio (1 mentions)
Diallyl trisulfide, by LKT Laboratories (1 mentions)
Hif 1α, by Santa Cruz Biotechnology (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Ly294002, by Beyotime (1 mentions)
Pentobarbital sodium, by Solarbio (1 mentions)
Cytochrome c, by Cell Signaling Technology (1 mentions)
Primary antibodies against cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Ex 527, by Merck Group (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Histone h3, by Abcam (1 mentions)
Gp91phox, by Santa Cruz Biotechnology (1 mentions)
Sodium carboxymethylcellulose cmc na, by Solarbio (1 mentions)
Ac foxo1, by Santa Cruz Biotechnology (1 mentions)
4 protocols using terminal deoxynucleotidyltransferase mediated dutp nick end labeling tunel assay kit
1
Diallyl Trisulfide Protects Cardiomyocytes
2
Evaluating Oxidative Stress and Apoptosis
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HKL and EX527 were obtained from Sigma-Aldrich (MO, USA). The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay kit was purchased from Roche Biochemicals (Mannheim, Germany). Kits for detecting lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD) activities, and malondialdehyde (MDA) content were purchased from the Institute of Nanjing Jiancheng Bio-Engineering Institute (Nanjing, Jiangsu, China). A primary antibody against gp91phox was obtained from Santa Cruz Biotechnology (CA, USA). Primary antibodies against cleaved caspase 3, Bcl-2, Bax, cytochrome c, and β-actin were all purchased from Cell Signaling Technology (Boston, MA, USA). Primary antibodies against SIRT1, Nrf2, heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO-1), and histone H3 were purchased from Abcam (Cambridge, MA, USA). Rabbit antigoat, goat antimouse, and goat antirabbit secondary antibodies were obtained from the Zhongshan Company (Beijing, China).
3
Oxidative Stress and Apoptosis Evaluation
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BBR, Evans blue (EB), triphenyltetrazolium chloride (TTC), 4′,6-diamino-2-phenylindole (DAPI), and protease inhibitor cocktail were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carboxymethylcellulose (CMC-Na) was purchased from Solarbio Technology (Beijing, China). Lactate dehydrogenase (LDH) assay kit, creatine kinase (CK), malondialdehyde (MDA), superoxide dismutase (SOD), superoxide generation, IL-6, and myeloperoxidase (MPO) assay kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). TNF-α ELISA kit was purchased from R&D Corporation (Minneapolis, USA). Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay kit was obtained from Roche Molecular Biochemicals (Mannheim, Germany). BCA protein quantification kit was purchased from Merck Millipore Technology (Darmstadt, Germany). The primary antibodies against SIRT1, Ac-Foxo1, gp91phox, caspase-3, Bcl-2, Bax, β-actin, and SIRT1 siRNA were purchased from Santa Cruz Biotechnology (CA, USA). Rabbit anti-goat, goat anti-rabbit, and goat anti-mouse secondary antibodies were purchased from the Zhongshan Company (Beijing, China).
4
Luteolin Modulates Autophagy and Apoptosis
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Luteolin (purity >98%) was purchased from Sigma-Aldrich (Fluka, Germany, Cat#: 491-70-3), dissolved in dehydrated alcohol as 50 mM stock solutions, and serially diluted in PBS immediately prior to experiments. The autophagy inhibitor 3-methyladenine (3-MA) was obtained from Sigma-Aldrich Co (St. Louis, MO, USA, Cat#: 5142-23-4). A Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan, Cat#: 341-07761). Ox-LDL was purchased from AbD Serotec (Kidlington, Oxford, UK). Oil red O (ORO), AO EB staining (Cat#: KGA213), MDC staining (Cat#: KGATG002) and the Annexin V-APC Apoptosis Detection Kit (Cat#: KGA214) were purchased from the KeyGEN Bioengineering Institute (Nanjing, China). A terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay kit was obtained from Roche (Mannheim, Germany, Cat#: 11767291910). Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM) were purchased from Gibco BRL (NY, USA). Antibodies against Bax (Cat#: ab10813), Bak (Cat#: ab32371) and ABCA1 (Cat#: ab18180) were purchased from Abcam (Cambridge, UK). Beclin-1 (Cat#: 3738), LC3 (Cat#: 12741), caspase-9 (Cat#: 9502), cleaved caspase-9 (Cat#: 9501), caspase-3 (Cat#: 9665), and cleaved caspase-3 (Cat#: 9661) antibodies were purchased from Cell Signaling Technology (MA, USA).
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