Total nuclear protein was extracted using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA). Total cellular protein was extracted using the radioimmune precipitation assay buffer (RIPA) (Santa Cruz Biotechnology Inc, Dallas, TX, USA) with 10% Protease Inhibitor Cocktail (Merck Millipore, Burlington, MA, USA). Western blot analysis was performed as described in [55 (link)]. Primary antibodies used and respective dilutions and incubation times are depicted in Table S3 . All blot images from direct Western Blot were captured by Bio-Rad ChemiDoc-Western Blot Digital Imaging System and the intensity of each band as well as respective ratio were quantified using Quantity One software (Bio-Rad Laboratories, Hercules, CA, USA), by comparing the protein band intensity with the loading control (β-Actin).
Chemidoc western blot digital imaging system
Manufactured by Bio-Rad
Sourced in United States
The ChemiDoc-Western Blot Digital Imaging System is a lab equipment product designed for the detection and analysis of chemiluminescent and fluorescent Western blots. It features a high-resolution camera and specialized software for image capture and quantification.
Automatically generated - may contain errors
2 protocols using chemidoc western blot digital imaging system
1
Quantification of Nuclear Protein Levels
2
Nuclear and Cytoplasmic Protein Extraction
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Total nuclear protein was extracted using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA). Total cellular protein was extracted using the radioimmune precipitation assay buffer (RIPA) (Santa Cruz Biotechnology Inc, Dallas, TX, USA) with 10% Protease Inhibitor Cocktail (Merck Millipore, Burlington, MA, USA). Western blot analysis was performed as described in [55] (link). Primary antibodies used and respective dilutions and incubation times are depicted in Table S3. All blot images from direct Western Blot were captured by Bio-Rad ChemiDoc-Western Blot Digital Imaging System and the intensity of each band as well as respective ratio were quantified using Quantity One software (Bio-Rad Laboratories, Hercules, CA, USA), by comparing the protein band intensity with the loading control (β-Actin).
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