Goat polyclonal secondary antibody to rabbit igg h l alexa fluor 488

Vero cells were washed twice with PBS and fixed with 4% paraformaldehyde for 15 min at 4 °C. The cells were permeabilized with 0.1% Triton X-100 on ice for 5 min. After washing 3 times with PBS, the cells were blocked with 3% BSA for 1 h at 37 °C. Subsequently, the cells were stained with an anti-PEDV monoclonal antibody (1:200, Medgene Lab, Brookings, SD, USA) at 4 °C for 12 h. The cells were then washed and incubated with Goat polyclonal Secondary Antibody to Rabbit IgG-H&L (Alexa Fluor^® 488) (1:200, Abcam, Cambridge, MA, USA) for 1 h at 37 °C. After 3 washes with PBS, the cells were incubated with DAPI (Sigma-Aldrich, St. Louis, MO, USA) at room temperature for 5 min and then examined with a fluorescence microscopy (Olympus CKX53, Tokyo, Japan).