The concentrations of Fe in Vero cells were measured using a flame atomic absorption spectrophotometer (AAS) (Hitachi, Japan). Iron stock standard solution (1 g/L) was purchased from Sigma-Aldrich, USA, and nitric acid (99%) from HiMedia Laboratories, India. Standard solutions were prepared by diluting 1 g/L iron solution with 1.5% HN03 solution at various concentrations. Samples were prepared at 10-fold dilution by diluting the homogenized cell samples with 1.5% HN03 solution. Deionized distilled water was used as blank. Standard solution was then mixed with distilled water, and a calibration curve was generated to estimate the levels of iron in the deionized distilled water. Following this, 40 mL of the sample preparation was injected into the atomic absorption spectrometry [51 (link)–54 (link)] in order to quantify iron in the Vero cells.
Nitric acid
Manufactured by HiMedia
Sourced in United States
Nitric acid is a colorless to yellowish highly corrosive liquid chemical compound. It is a strong oxidizing agent and is widely used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ferric chloride, by HiMedia (1 mentions)
Hexane, by HiMedia (1 mentions)
Glacial acetic acid, by HiMedia (1 mentions)
Sodium phosphate, by HiMedia (1 mentions)
Aluminum chloride, by HiMedia (1 mentions)
Ammonium molybdate, by HiMedia (1 mentions)
Sulfuric acid, by HiMedia (1 mentions)
Chloroform, by HiMedia (1 mentions)
Hydrochloric acid (hcl), by HiMedia (1 mentions)
Ferrous sulfate, by HiMedia (1 mentions)
Folin ciocalteu reagent, by HiMedia (1 mentions)
Sodium hydroxide (naoh), by HiMedia (1 mentions)
Ethanol, by HiMedia (1 mentions)
Methanol, by HiMedia (1 mentions)
Sodium carbonate, by HiMedia (1 mentions)
2 protocols using nitric acid
1
Quantifying Iron in Vero Cells
2
DPPH Antioxidant Activity Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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1,1-Diphenyl-2-picrylhydrazyl (DPPH), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), nitro blue tetrazolium chloride (NBT), hydrogen peroxide (H 2 O 2 ), nicotinamide adenine dinucleotide phosphate (NADPH), ribo avin, nicotinamide adenine dinucleotide (NADH), methionine, phenazine methosulfate (PMS), Triton X-100, guaiacol, glutathione, ascorbic acid, gallic acid, rutin, and sodium salicylate were provided by Sigma-Aldrich, Merck. Ferrous sulfate (FeSo 4 ), Tris HCl, sodium phosphate, ammonium molybdate, ferric chloride (FeCl 3 ), sodium carbonate, Folin-Ciocalteu reagent, sodium carbonate (Na 2 CO 3 ), ammonia solution (NH 3 ), sulfuric acid (H 2 SO 4 ), hydrochloric acid (HCl), Fehling's solutions A and B, aluminum chloride (AlCl 3 ), mercury potassium iodide, sodium hydroxide (NaOH), isoamyl alcohol, glacial acetic acid, nitric acid, methanol, chloroform, ethanol and hexane were purchased from Himedia.
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