hPXR (H-11) sc-48340 mouse monoclonal antibody and relevant horseradish peroxidase (HRP)-labeled secondary antibodies were purchased from Santa Cruz Biotechnology Inc., (Santa Cruz, CA, USA). β-actin AP0060 rabbit antibody was from Bioworld Technology, Inc. (Minneapolis, MN, USA). SR12813, dissolved in dimethyl sulfoxide (DMSO), and 4-hydroxytamoxifen were purchased from Sigma-Aldrich (St. Louis, MO, USA). Docetaxel was purchased from Qilu Pharmaceutical Co., Ltd (Jinan, China). The Cell Counting Kit-8 (CCK-8) cell proliferation-toxicity test kits were from Dojindo (Kumamoto, Japan). An RNA extraction kit, reverse transcription system and semi-quantitative PCR reagents were purchased from Takara Bio Inc. (Shiga, Japan). The total protein extraction kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China). The enhanced chemiluminescence (ECL) detection system was purchased from Millipore (Billerica, MA, USA). The 7300 Real Time PCR system was from ABI (Warrington, UK). The Microplate Reader system was from Promega (Madison, WI, USA) and the BD FACSCalibur Flow Cytometer was purchased from Becton-Dickinson (New York, NY, USA).
Enhanced chemiluminescence ecl detection system
Manufactured by Merck Group
Sourced in United States
The Enhanced chemiluminescence (ECL) detection system is a laboratory equipment used to detect and measure protein expression levels in biological samples. It utilizes chemiluminescent reactions to generate a light signal that is proportional to the amount of target protein present. The system provides a sensitive and quantitative method for analyzing protein samples.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Total protein extraction kit, by Beyotime (1 mentions)
Reverse transcription system, by Takara Bio (1 mentions)
Rna extraction kit, by Takara Bio (1 mentions)
Sr12813, by Merck Group (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
4 hydroxytamoxifen, by Merck Group (1 mentions)
Docetaxel, by Qilu Pharmaceutical (1 mentions)
Active caspase 3, by Cell Signaling Technology (1 mentions)
Lycorine, by Yuanye Bio-Technology (1 mentions)
4e bp1, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
P mtor, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Caspase 3, by Proteintech (1 mentions)
3 protocols using enhanced chemiluminescence ecl detection system
1
Characterization of hPXR Signaling
2
Lycorine-Induced Apoptosis Signaling
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Lycorine (purity > 98%) was purchased from Shanghai Yuanye Bio-Technology (Shanghai, China). A 40 mM stock solution was prepared in Dimethyl sulfoxide (DMSO) and stored at -20°C in aliquots. Dimethyl sulfoxide (DMSO) was purchased from Sigma (St. Louis, MO, USA). RPMI-1640 medium, fetal calf serum (FCS), L-glutamine were purchased from GIBCO Invitrogen Life Technologies (Carlsbad, USA). Primary antibodies against AKT, p-AKT, mTOR, p-mTOR, 4EBP1, Bax, Bcl-2, Active caspase-3 and actin were purchased from Cell Signaling Technology, Inc. (Beverly, USA). The enhanced chemiluminescence (ECL) detection system was obtained from Millipore. (Millipore, USA).
3
Investigating PTEN-AKT Pathway in Melanoma
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Chemicals and reagents. CTD and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). RPMI-1640 medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA), fetal calf serum (FCS) and L-glutamine were purchased from Gibco (Thermo Fisher Scientific, Inc.). Primary antibodies against PTEN, protein kinase B (AKT), protein kinase B (p) AKT, Bax, B-cell lymphoma-2 (bcl-2), caspase-3, anti-GAPDH and the appropriate secondary antibodies were purchased from ProteinTech Group, Inc. (Chicago, IL, USA). The enhanced chemiluminescence (ECL) detection system was obtained from Millipore (EMD Millipore, Billerica, MA, USA).
Cell culture. The A375 human melanoma cancer cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured at 37˚C in a 5% CO 2 humidified incubator in RPMI-1640 medium supplemented with 10% heat-inactivated FCS, 2 mM glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin.
Cell culture. The A375 human melanoma cancer cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured at 37˚C in a 5% CO 2 humidified incubator in RPMI-1640 medium supplemented with 10% heat-inactivated FCS, 2 mM glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin.
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