Water samples were collected monthly from May 2018 to April 2019 at a pelagic station (water depth, ca. 73 m) on Lake Biwa (35°13′09.5″N, 135°59′44.7″E) from two water depths, representing the epilimnion (5 m) and the hypolimnion (65 m) (24 samples in total). Vertical profiles of chlorophyll a concentration, temperature, and dissolved oxygen were collected using a RINKO CTD profiler (ASTD102; JFE Advantech). The collected lake water was immediately sequentially filtered through a 200-μm mesh, 5-μm polycarbonate filter (TMTP14250; Merck Millipore) and a 0.22-μm-pore-size Sterivex cartridge (SVGP01050; Merck Millipore), using a peristaltic pump system onboard. Filtration was performed until the Sterivex cartridge was clogged (1 to 2.5 L of lake water was filtered for each cartridge), and at least four Sterivex cartridges were collected for each sample. The filters were flash-frozen in a dry ice-ethanol bath, transported to the laboratory on dry ice, and stored at −80°C until further processing. Water samples were collected between 8:00 a.m. and 11:00 a.m. on each sampling day and processed to the freezing step within 1 h after collection. Prokaryotic cell abundance was determined for each sample using a flow cytometer (CytoFLEX; Beckman Coulter) following fixation of the water sample with 1% glutaraldehyde and staining with 0.25× SYBR green solution (S7563; Invitrogen).
Tmtp14250
Manufactured by Merck Group
TMTP14250 is a laboratory equipment product from Merck Group. It is designed for specific laboratory functions, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tmtp14250
1
Limnological Sampling of Lake Biwa
2
Seasonal Monitoring of Microbial Community in Lake Biwa
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Water samples were collected monthly from May 2018 to April 2019 at a pelagic station (water depth ca. 73 m) on Lake Biwa (35°13′09.5″ N, 135°59′44.7″ E) from two water depths, representing the epilimnion (5 m) and hypolimnion (65 m) (24 samples in total). Vertical profiles of chlorophyll-a concentration, temperature, and dissolved oxygen were collected using a RINKO CTD profiler (ASTD102; JFE Advantech). The collected lake water was immediately sequentially filtered through a 200 µm mesh, 5 µm polycarbonate filter (TMTP14250; Merck Millipore), and 0.22 µm pore Sterivex cartridge (SVGP01050; Merck Millipore), using a peristaltic pump system onboard. Filtration was performed until the Sterivex cartridge was clogged (1-2.5 liters of lake water were filtered for each cartridge), and at least four Sterivex cartridges were collected for each sample. The filters were flashfrozen in a dry-ice ethanol bath, transported to the laboratory on dry ice, and stored at -80°C until further processing. Water samples were collected between 8:00 am and 11:00 am on each sampling day and processed to the freezing step within 1 h after collection. Prokaryotic cell abundance was determined for each sample using a flow cytometer (CytoFLEX; Beckman Coulter) following fixation of the water sample with 1% glutaraldehyde and staining with 0.25× SYBR Green solution (S7563; Invitrogen).
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