Horse serum

Manufactured by Cytiva

Sourced in United States

Horse serum is a biological product derived from the blood of horses. It is a complex mixture of proteins, growth factors, and other biomolecules that can be used in various cell culture and laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (7 mentions) Fetal bovine serum (fbs), by Cytiva (5 mentions) Penicillin, by Thermo Fisher Scientific (3 mentions) Streptomycin, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Cytiva (2 mentions) Anti myosin heavy chain antibody, by Merck Group (1 mentions) Hepes, by Cytiva (1 mentions) Sodium pyruvate, by Cytiva (1 mentions) Ti s inverted fluorescence microscope, by Nikon (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Cytiva (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Hematoxyline, by Merck Group (1 mentions) Pantothenic acid, by Merck Group (1 mentions) Streptomycin, by Welgene (1 mentions) Fluoromount aqueous mounting media, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions)

18 protocols using horse serum

Differentiation of C2C12 Myoblasts into Myotubes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse C2C12 myoblasts were purchased from Cyagen Biosciences, Inc. Human umbilical vein endothelial cells (HUVECs), PUMC-HUVEC-T1, were kindly provided by the Molecular Biology Laboratory of PLA General Hospital (purchased from the National Experimental Cell Resource Sharing Platform). C2C12 myoblasts and PUMC-HUVEC-T1 cells were maintained in DMEM high sugar culture medium (Hyclone; Cytiva) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 U/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37˚C, 5% CO₂. To induce myotube formation, C2C12 myoblasts with 60-80% confluence were seeded in DMEM differentiation medium containing 2% horse serum (Cytiva), 100 U/ml penicillin and 100 U/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37˚C for 5 days (19 (link)). The success of differentiation was validated by Giemsa staining of myotubes (cat. no. G4507; Sigma-Aldrich, Merck KGaA) and western blotting using anti-myosin heavy chain antibody (cat. no. sc-53095, 1:1,000, Santa Cruz Biotechnology).
For Giemsa staining, cells were fixed in 100% methanol at room temperature (RT) for 10 min. After air dry, Giemsa stain was added and stained for 30 min at RT. After rinsing with deionized water, cells were examined at x20 magnification under a light microscope (DP73; Olympus Corporation).

Jia L., Zheng P., Wang H., Kang L., Wu H, & Fu X. (2022). VEGF alleviates lower limb ischemia in diabetic mice by altering muscle fiber types. Experimental and Therapeutic Medicine, 23(4), 251.

+ Open protocol

+ Expand

Derivation of Murine Bone Marrow-Derived Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow cells (BMs) were flushed from humerus, femur and tibia of 6-8 weeks old C57BL/6 mice. To obtain BMDMs, BMs were cultured in tissue culture non-treated petri dish (Hycon, Thailand) with 8 ml of DMEM complete media (Cytiva, UK) supplemented with 10% (v/v) Fetal Bovine Serum (FBS) (Gibco, USA), 10 mM HEPES (Cytiva), 1 mM sodium pyruvate (Cytiva) and 1x Penicillin/Streptomycin G (Cytiva), supplemented with 5% (v/v) horse serum (Cytiva), and 20% (v/v) L929-conditioned media. On day 4, three ml of fresh media supplemented with 20% L929-conditioned media and 5% horse serum was added to the culture. Cells were harvested on day 7 using ice-cold PBS. Macrophage phenotype was confirmed by flow cytometry by staining using mouse anti-F4/80 and CD11b antibodies (BioLegend, USA). The derived BMDMs were seeded at 5×10⁴ cells/well in 200 µl of DMEM complete media without horse serum and L929-conditioned media in 96-well plate before transfection.

Sittplangkoon C., Alameh M.G., Weissman D., Lin P.J., Tam Y.K., Prompetchara E, & Palaga T. (2022). mRNA vaccine with unmodified uridine induces robust type I interferon-dependent anti-tumor immunity in a melanoma model. Frontiers in Immunology, 13, 983000.

+ Open protocol

+ Expand

Neuronal Differentiation of PC-12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

PC-12 is a classic neuronal cell line. Studies have suggested that NGF can induce PC-12 cell differentiation (26-28 (link)). Thus, undifferentiated PC12 cells (The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences) were cultured in RPMI-1640 medium (cat. no. SH30809.018; Hyclone; Cytiva) supplemented with 10% horse serum (cat. no. 26050088; Gibco; Thermo Fisher Scientific, Inc.) and 5% FBS, and were treated with 10, 50 and 100 ng/ml NGF cell supernatants from NGF-overexpressing MDA-MB231 cells at 37˚C. NGF was obtained from the supernatants of NGF-overexpressing cells and the concentrations were confirmed by ELISA (Human NGF/NGF-β ELISA kit; cat. no. EK0469; Boster Biological Technology). NGF-β standards was used to establish the standard curve. The cell number and axon length were calculated by fluorescence microscopy (NIKON Ti-s inverted fluorescence microscope; Nikon Corporation); 250 cells from five fields of vision (n=50) were analyzed.

Liu Y., Zou L., Wang P., Zhou J., Yuan C, & Wang J. (2021). Construction of differential expression plasmids of NGF to detect its influence on PC12 cell neuronal differentiation. Experimental and Therapeutic Medicine, 21(4), 363.

+ Open protocol

+ Expand

Cell Culture and Virus Propagation

Check if the same lab product or an alternative is used in the 5 most similar protocols

A549 cells U2OS cells (Gifts from Dr. Renjin Chen, Xuzhou Medical University, Xuzhou, China) were maintained in DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (HyClone Laboratories, Logan, UT, USA). MDBK cells (Purchased from Chinese model culture preservation center, Shanghai, China) were maintained in DMEM supplemented with 10% horse serum (HyClone Laboratories, Logan, UT, USA). BoHV-1 (Colorado1 stain) was propagated in MDBK cells. Aliquots of virus stocks were stored at −70 °C until use.

Zhu L., Fu X., Yuan C., Jiang X, & Zhang G. (2018). Induction of Oxidative DNA Damage in Bovine Herpesvirus 1 Infected Bovine Kidney Cells (MDBK Cells) and Human Tumor Cells (A549 Cells and U2OS Cells). Viruses, 10(8), 393.

+ Open protocol

+ Expand

BoHV-1 Virus Inactivation Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDBK (Madin-Darby bovine kidney) cells (kindly provided by Dr. Leonard J. Bello, University of Pennsylvania) were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% horse serum (HyClone Laboratories, Logan, UT, USA). BoHV-1 of Colorado1 stain (kindly provided by Dr. Leonard J. Bello, University of Pennsylvania) was propagated in MDBK cells. Aliquots of virus stocks were stored at −70 °C until use. The inactivation of the BoHV-1 virus with UV (ultraviolet) irradiation was performed as previously described [25 (link)]. Complete inactivation of the virus was characterized by plaque assay in MDBK cells.

Zhu L., Jiang X., Fu X., Qi Y, & Zhu G. (2018). The Involvement of Histone H3 Acetylation in Bovine Herpesvirus 1 Replication in MDBK Cells. Viruses, 10(10), 525.

+ Open protocol

+ Expand

T-cell Leukemia and Lymphoma Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human T cell leukemia cell line Jurkat (clone E6-1) cells were obtained from the American Typing Culture Collection (ATCC, Manassas, VA). Human T lymphoblastic leukemia Molt4-Bcl2 and Molt4-hyg, derived through stable transfection of the expression vector pMEP with and without full length murine Bcl2, respectively, were provided by Dr. Y.A. Hannun (Department of Medicine, Duke University Medical Center, Durham, NC) [29 (link)]. DEL, an ALK-positive anaplastic large-cell lymphoma, was obtained from Dr. S. Mathas (Medical University, Berlin, Germany). Fibrosarcoma Ht1080 and Ht1080mut containing wild type p53 and mutant p53 were obtained from Dr. A. Merrick, NIEHS. All cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (Atlanta Biologicals, Atlanta, GA), 2 mM L-glutamine, penicillin, and streptomycin (Gibco). The T-cell hybridoma cell line DO11.10 was provided by Dr. B.A. Osborne (University of Massachusetts, Amherst, MA) and grown in RPMI-1640 medium supplemented with 10% horse serum (Hyclone Laboratories, Logan, UT). Farnesol was dissolved in DMSO and cells were treated with farnesol at the concentrations indicated or with vehicle control (0.1% DMSO final concentration).

Joo J.H., Ueda E., Bortner C.D., Yang X.P., Liao G, & Jetten A.M. (2015). Farnesol activates the intrinsic pathway of apoptosis and the ATF4-ATF3-CHOP cascade of ER stress in human T lymphoblastic leukemia Molt4 cells. Biochemical pharmacology, 97(3), 256-268.

+ Open protocol

+ Expand

Differentiation of Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s modified Eagle’s medium (DMEM, high glucose), penicillin and streptomycin (PS) were purchased from Welgene (Dalseogu, Daegu, Korea), whereas fetal bovine serum (FBS) and horse serum are respectively from hyclone laboratories Inc (Logan, UT, USA) from Gibco Technologies Inc (Grand Island, NY, USA). Retinoic acid, linolenic acid (LA), dexamethasone, insulin, biotin, ascorbic acid, pantothenic acid, ascorbic acid, paraformaldehyde, hematoxyline, eosin, Triton X-100, 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and fluoromount aqueous mounting media were obtained from Sigma Aldrich (St. Louis, MO, USA). Further, primary and secondary antibodies of both Donkey anti-goat immunoglobulin labelled with fluorescein isothiocyanate (IgG-FITC) and donkey anti-goat IgG-biotinylated were purchased from Santacruz biotechnology (Dallas, TX, USA) and primers from Macrogen.

Sugathan S., Lee S.J., Shiwani S, & Singh N.K. (2019). Transdifferentiation of bovine epithelial cells towards adipocytes in the presence of myoepithelium. Asian-Australasian Journal of Animal Sciences, 33(2), 349-359.

+ Open protocol

+ Expand

BoHV-1 Propagation in MDBK Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDBK cells were cultured in DMEM (Gibco BRL) containing 10% horse serum (HyClone Laboratories, Logan, UT, USA). BoHV-1 (Colorado1 stain) was propagated in MDBK cells. Aliquots of the virus stocks were tittered in MDBK cells and stored at −80°C.

Fu X., Chen D., Ma Y., Yuan W, & Zhu L. (2019). Bovine Herpesvirus 1 Productive Infection Led to Inactivation of Nrf2 Signaling through Diverse Approaches. Oxidative Medicine and Cellular Longevity, 2019, 4957878.

+ Open protocol

+ Expand

C2C12 Myoblast Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

C2C12 myoblasts (CRL-1772; ATCC, Manassas, VA, USA) were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS; both from HyClone Laboratories, Inc., Logan, UT, USA) at 37°C with 5% CO₂. To induce myogenic differentiation, the cells were plated on tissue culture plates and grown to 95% confluence before switching to differentiation medium (DM) (DMEM and 2% horse serum; HyClone Laboratories, Inc.). The cells were replenished with fresh DM every other day until day 5.

Zhong Y., Zou L., Wang Z., Pan Y., Dai Z., Liu X., Cui L, & Zuo C. (2016). Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation. International Journal of Molecular Medicine, 38(5), 1411-1418.

+ Open protocol

+ Expand

Inactivation of BoHV-1 Virus by UV Irradiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDBK cells and RS-1 cells were maintained in DMEM (Gibco BRL) supplemented with 10% horse serum and fetal bovine serum (HyClone Laboratories, Logan, UT, USA), respectively. BoHV-1 (Colorado1 stain) was propagated in MDBK cells. Aliquots of virus stocks were stored at −70 °C until use. The virus was titrated in MDBK cells with results expressed as TCID₅₀/mL calculated using the Reed-Muench formula.
To inactivate BoHV-1 virus with UV-irradiation, virus stock was dispersed into 100-mm tissue culture dishes, and directly placed under a UV lamp (20 W) for 30 min. Complete inactivation of the virus was confirmed by the fact that no plaque was produced in MDBK cells exposed to the UV treated virus for 48 h.

Zhu L., Yuan C., Ding X., Jones C, & Zhu G. (2017). The role of phospholipase C signaling in bovine herpesvirus 1 infection. Veterinary Research, 48, 45.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!

Request a quote for « Horse serum »