H1355

Manufactured by American Type Culture Collection

Sourced in United States

H1355 is a laboratory equipment product designed for general laboratory use. It provides a stable and controlled environment for various applications. The core function of H1355 is to maintain specific temperature, humidity, and atmospheric conditions as required by the user's application.

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Lab products found in correlation

18 protocols using h1355

Establishment and Characterization of NSCLC and Murine Lung Cancer Cell Lines

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Human NSCLC cell lines (A549, H358, H460, H23, H1792, H1975, H1355, and H1299) and NIH/3T3 cell line were purchased from ATCC. All NSCLC cell lines were grown in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (10,000 U/mL). NIH/3T3 cells were grown in Dulbecco’s modified Eagle medium (DMEM) with 10% FBS and 1% penicillin/streptomycin. To establish murine lung cancer cell lines, 4 to 10 weeks after Ad-Cre infection, lung tumor nodules of KP mice were collected, minced, and dissociated with Trypsin LE. The cell lines were established after eight passages with complete removal of fibroblasts. Murine lung cancer cell lines (KP cell lines) were maintained in DMEM with 10% FBS and 1% penicillin/streptomycin (10,000 U/mL). Three murine KP cell lines (KP836, KP952, and KP944) were derived from three different KP mice. Genotyping of KP cell lines was confirmed by genomic DNA PCR as previously described (DuPage et al., 2009 (link)) (See Figure S3A).

Kim M.J., Cervantes C., Jung Y.S., Zhang X., Zhang J., Lee S.H., Jun S., Litovchick L., Wang W., Chen J., Fang B, & Park J.I. (2021). PAF remodels the DREAM complex to bypass cell quiescence and promote lung tumorigenesis. Molecular Cell, 81(8), 1698-1714.e6.

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Cell Lines Maintenance Protocol

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H2110, H1915, H650, H1623, H2126, HCC827, A549, H810, H1048, H1355 and HEK293T, and Beas‐2B were purchased from ATCC. The H157 cell line was a kind gift from Dr Phillip Dennis (Johns Hopkins University). All cell lines were maintained in RPMI1640 media (Invitrogen) supplemented with 2 mM L‐glutamine (Gibco), 10% FBS (Lonza), and 1% Pen/Strep (Gibco). 293FT cells (Invitrogen) were maintained in DMEM media (Gibco) supplemented with 10% FBS (Lonza) 4 mM L‐glutamine (Gibco), 1 nM sodium pyruvate (Gibco), and 0.1 mM NEAA (Gibco). All experiments were performed within 6 months of cell line delivery and cell lines were authenticated by DNA sequencing at the start of experiment. After 6 months, cells were discarded and new cells ordered from ATCC. Mycoplasma testing was performed on regular basis.

Testoni E., Stephenson N.L., Torres‐Ayuso P., Marusiak A.A., Trotter E.W., Hudson A., Hodgkinson C.L., Morrow C.J., Dive C, & Brognard J. (2016). Somatically mutated ABL1 is an actionable and essential NSCLC survival gene. EMBO Molecular Medicine, 8(2), 105-116.

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Establishing MART-1 expressing cell lines

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Cell lines H1355, H1666, H157, H322, H1975, HCC2935, and T2 were obtained from ATCC. PC9 cells were obtained from the European Collection of Authenticated Cell Cultures. All cell lines were cultured in RPMI medium with GlutaMAX, penicillin, streptomycin, and 10% fetal bovine serum. MART-1–expressing HCC2935 and PC9 cells were generated via lentiviral transduction. Briefly, MART-1 expression plasmid was synthesized by GeneArt (Thermo Fisher Scientific) and subcloned into pCDH1-CMV-MCS-EF1-GFP-T2a-puro lentiviral vector (System Biosciences). 293X cells (internal cell bank) were transfected with pPACKH1 and lentiviral vector using 293Fectin (Gibco). Lentiviral supernatant was harvested and concentrated 48 hours after transfection. PC9 and HCC2935 cells were transduced through centrifugation with concentrated lentivirus and polybrene (MilliporeSigma). The transduced cells were cultured in medium supplemented with puromycin (Gibco) for at least 1 week to establish stable cell lines.

Tu E., McGlinchey K., Wang J., Martin P., Ching S.L., Floc’h N., Kurasawa J., Starrett J.H., Lazdun Y., Wetzel L., Nuttall B., Ng F.S., Coffman K.T., Smith P.D., Politi K., Cooper Z.A, & Streicher K. (2022). Anti–PD-L1 and anti-CD73 combination therapy promotes T cell response to EGFR-mutated NSCLC. JCI Insight, 7(3), e142843.

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Establishment of Human and Murine Lung Cancer Cell Lines

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Establishing Adenocarcinoma Cell Lines for Research

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The human lung adenocarcinoma (ADC) cell lines, H928, H1355, A549, and human lung large cancer cell line, H1299 were purchased from ATCC. CL1-0 and CL1-5 were kind gifts from Dr. Pan-Chyr Yang’s Lab^{11 (link)}. All cells were incubated under the procedure as previous described^{9 (link)}. For establishing stable cell lines, the pGIPZ lentiviral shRNA system (Thermo, MA, USA) was used with the BZW1 sequence. Lentivirus was used to infect CL1-5 cells for two days. We selected stable clones with 1 μg/ml puromycin (Sigma-Aldrich, MO, USA) for 14 days. The methods followed the procedure in our lab^{10 (link)}.

Chiou J., Chang Y.C., Jan Y.H., Tsai H.F., Yang C.J., Huang M.S., Yu Y.L, & Hsiao M. (2019). Overexpression of BZW1 is an independent poor prognosis marker and its down-regulation suppresses lung adenocarcinoma metastasis. Scientific Reports, 9, 14624.

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Authenticated Cancer Cell Line Cultivation

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The human cancer cell lines H1975, HCC827, H2122, CHO, CHL and H1355 were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). PC9 cell line was purchased from the Sigma-Aldrich (St. Louis, MO, USA). A549, H3255 were purchased from Cobioer Biosciences CO., LTD (Nanjing, China). H1975, PC-9, HCC827 and EGFR mutant isogenic BaF3 cells were cultured in RPMI 1640 media (Corning, USA) with 10% fetal bovine serum (FBS) and supplemented with 2% L-glutamine and 1% penicillin/streptomycin. A549 and H1355 were cultured in F-12K Nutrient Mixture (kaighn's Modification) (Gibco, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep. H3255 was cultured in BEGM media (LONZA, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep.
We have authenticated the following cell lines through cell line short tandem repeat (STR) profiling (GENEWIZ, Suzhou, China): H1975, PC9, H3255, HCC827, A549, H2122, H1703. All cell lines matched >90% with lines listed in the ATCC, DSMZ or JCRB Cell Line Bank STR Profile Information.

Hu C., Wang A., Wu H., Qi Z., Li X., Yan X.E., Chen C., Yu K., Zou F., Wang W., Wang W., Wu J., Liu J., Wang B., Wang L., Ren T., Zhang S., Yun C.H., Liu J, & Liu Q. (2017). Discovery and characterization of a novel irreversible EGFR mutants selective and potent kinase inhibitor CHMFL-EGFR-26 with a distinct binding mode. Oncotarget, 8(11), 18359-18372.

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Cell Line Characterization and Authentication

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A549, H441, H23, H358 and H1355 cells were obtained from the American Type Culture Collection (ATCC) and cultured as described. CL1-5 cells were kindly provided by Dr. P.-C. Yang (Department of Internal Medicine, National Taiwan University Hospital, Taiwan). These cells were cultured and stored according to the suppliers’ instructions and used at passages 5 to 20. Once resuscitated, cell lines are routinely authenticated (once every 6 months, cells were last tested in June 2015) through cell morphology, proliferation rate, a panel of genetic markers, and contamination checks.

Chen M.J., Wu D.W., Wang Y.C., Chen C.Y, & Lee H. (2016). PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness. Scientific Reports, 6, 34933.

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Lung Adenocarcinoma Cell Line Maintenance and Genetic Manipulation

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Human lung adenocarcinoma cell lines, (H1355, H1573, H23, A549, H1975, and H441) were purchased from the American Type Culture Collection (ATCC) cell bank. The lung adenocarcinoma cell lines CL1-0 and CL1-5 were established and provided as a gift from Dr. Pan-Chyr Yang (National Taiwan University, Taipei, Taiwan). H1355, H1573, H23, CL1-0, H1975, H441, and CL1-5 cells were maintained in RPMI 1640 medium, supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA). The human lung adenocarcinoma cell line A549 was grown in F12K medium plus 10% FBS (Invitrogen) at 37 °C in a humidified atmosphere of 5% CO₂. For establishing the stable PGK1 or HTATSF1 cell lines, the pGIPZ lentiviral shRNAmir system (Thermo, Waltham, MA, USA) was used with PGK1 and HTATSF1 sequences, respectively. The lentiviruses were used to infect CL1-5 cells for 2 days. Stable clones were selected with puromycin (Sigma-Aldrich, St. Louis, MO, USA) at 1 μg/ml for 2 weeks.

Chang Y.C., Chan M.H., Li C.H., Yang C.J., Tseng Y.W., Tsai H.F., Chiou J, & Hsiao M. (2021). Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1. Cell Death Discovery, 7, 135.

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Lung Adenocarcinoma Cell Lines Characterization

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A549, H23, H358, H441, H1355 and H1975 lung adenocarcinoma cells were obtained from the American Type Culture Collection (ATCC) and cultured as described previously [31 (link)]. CL-3, CL1-0 and CL1-5 cells were kindly provided by Dr. P. C. Yang (Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan). TL-4, TL-5, TL-6 TL-10 and TL-13 lung adenocarcinoma cells were established from pleural effusions from Taiwanese lung cancer patients and cultured as described previously [32 (link), 33 (link)].

Chen P.M., Wu T.C., Cheng Y.W., Chen C.Y, & Lee H. (2015). NKX2-1-mediated p53 expression modulates lung adenocarcinoma progression via modulating IKKβ/NF-κB activation. Oncotarget, 6(16), 14274-14289.

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Cell Culture and Transfection Protocol

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HEK293T and HEK293A cells were purchased from the American Type Culture Collection and cultured in Dulbecco modified essential medium supplemented with 10% fetal bovine serum at 37 °C in 5% CO₂ (v/v). A549, H1666, H1792, H460, H1299, H1355, H1975, H23, H187 were purchased from the American Type Culture Collection and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum at 37 °C in 5% CO₂ (v/v). The culture media contained 1% penicillin and streptomycin. All the cells were passed the test of mycoplasma. Plasmid transfection was performed with polyethyleneimine reagent, as reported previously^{31 (link)}.

Wang C., Chen Z., Nie L., Tang M., Feng X., Su D., Zhang H., Xiong Y., Park J.M, & Chen J. (2020). Extracellular signal-regulated kinases associate with and phosphorylate DHPS to promote cell proliferation. Oncogenesis, 9(9), 85.

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