Anaesthetised flies were immobilized at the end of a pipette tip by applying a drop of colourless nail polish on the proboscis. For recordings, GC 100F-10 borosilicate glass capillaries (640786, Harvard Apparatus, MA) were pulled to form electrodes and then filled with 0.8% (w/v) NaCl. The reference electrode was placed on the centre of the eye and the ground electrode on the thorax to obtain voltage changes post stimulation. The protocol for recording involved dark adapting the flies for 5 minutes initially, following which they were shown green flashes of light for 2 secs (10 times), and 12 secs of recovery time in dark between the two flashes. Voltage changes obtained were amplified using DAM50 amplifier (SYS-DAM50, WPI, FL), and recorded using pCLAMP10.7. Analysis was done using Clampfit 10.7 (Molecular Devices, CA). For analysis, the average of 10 recordings was taken for per fly.
Gc 100f 10 borosilicate glass capillaries
Manufactured by Harvard Apparatus
Sourced in United States
The GC 100F-10 borosilicate glass capillaries are laboratory equipment designed for general use. They are made of high-quality borosilicate glass and are intended for a variety of applications that require thin, cylindrical glass components.
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Lab products found in correlation
2 protocols using gc 100f 10 borosilicate glass capillaries
1
Electrophysiological Recordings from Anaesthetized Flies
2
Electroretinography in Anaesthetised Flies
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Anaesthetised flies were immobilized at the end of a pipette tip by applying a drop of colourless nail polish on the proboscis. For recordings, GC 100F-10 borosilicate glass capillaries (640786, Harvard Apparatus, MA, USA) were pulled to form electrodes and then filled with 0.8% (w/v) NaCl. The reference electrode was placed on the centre of the eye and the ground electrode on the thorax to obtain voltage changes post stimulation. The protocol for recording involved dark adapting the flies for 5 min initially, following which they were shown green flashes of light for 2 s (ten times), and 12 s of recovery time in dark between the two flashes. Voltage changes obtained were amplified using DAM50 amplifier (SYS-DAM50, WPI, FL, USA), and recorded using pCLAMP10.7. Analysis was done using Clampfit 10.7 (Molecular Devices, CA, USA). For analysis, the average of ten recordings was taken per fly.
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