Mmp 3

Complete Freund’s adjuvant (CFA) was obtained from Chondrex, Inc. (USA). D-luciferin was purchased from Abcam (UK). Rabbit anti-BMP3, anti-TNF-a, and anti-TIMP metallopeptidase inhibitor 1 (TIMP-1) antibodies were obtained from Abcam (UK). Rabbit anti-MMP-3 and anti-MMP-9 antibodies were acquired from Merck Millipore (USA). Rabbit anti-IL-6, anti-IL-1β, and anti-IL-17A were purchased from Bioworld (USA). The rat anti-β-actin antibody was purchased from CST (USA). A horseradish peroxidase (HRP)-labeled goat anti-rabbit immunoglobulin (IgG) was procured from Zhongshan Biotechnology Corporation (China). BMP3, MMP-3, MMP-9, TIMP-1, TNF-a, IL-6, IL-1β, IL-17A, CCL-2, CCL-3, VCAM-1, and β-actin primers were synthesized by the Shanghai Sangon Biological and Technological Company (China).

Rabbit anti-MMP3, anti-MMP9, and anti-METTL3 antibodies were obtained from Abcam (Cambridge, UK). We obtained the rabbit anti-phospho-(p)-p65 antibody, anti-β-actin antibody, anti-vimentin antibody, and anti-p65 antibody from Cell Signaling Technology, Inc. (Danvers, MA, USA). Rabbit anti-proliferating cell nuclear antigen (PCNA) antibody was obtained from Bioss (Beijing, China). The secondary antibody we used was from Zhongshan Biotechnology Corporation (Beijing, China). The cell cycle kits we used were from BestBio (Shanghai, China). The enzyme-linked immunosorbent assay (ELISA) kits for IL-6 were purchased from Elabscience Biotechnology Co., Ltd. METTL3, MMP3, MMP9, PCNA, IL-6, IL-1β, and TNF-α primers were synthesized by Shanghai Sangon Biological and Technological Company (Shanghai, China).