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Hek 293 crl 1573 cells

Manufactured by American Type Culture Collection
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HEK 293 (CRL-1573™) cells are a widely used immortalized cell line derived from human embryonic kidney cells. These cells are commonly used in various research applications, including protein expression, virus production, and gene delivery studies.

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Lab products found in correlation

Chymostatin, by Merck Group (1 mentions) Alprenolol, by Merck Group (1 mentions) Isoproterenol, by Merck Group (1 mentions) Anti β actin antibody sc 47778, by Santa Cruz Biotechnology (1 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions) Thp 1 tib 202 cells, by American Type Culture Collection (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Fungizone, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CRL-1573 (100 citations) 293 cells (35 citations) HEK-293 (CRL-1573) (22 citations) HEK-293 cells (9 citations)

5 protocols using hek 293 crl 1573 cells

1

Anti-FLAG and Anti-SAP97 Antibody Protocol

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Anti-FLAG (M2) Cy3-labeled antibody was from Sigma (St. Louis MO). Anti-SAP97 antibody from Stressgen (Enzo Life Sciences, Farmingdale, NY), anti-SNX27 (ab77799) from Abcam (Cambridge MA). Anti β-actin antibody (sc-47778) from Santa Cruz Biotechnology (Santa Cruz, CA). Myristoylated PKA inhibitory peptide, 14–22 amide myristoylated Myr-N-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-NH2 (PKI) was purchased from Calbiochem (EMD-Millipore, Billerica MA). Isoproterenol, alprenolol, dithiothreitol, poly-L-lysine (P0899), leupeptin, aprotinin, chymostatin and phenyl methyl sulfonyl fluoride were from Sigma Chemical Co. (St. Louis, MO). Flexi siRNA oligo sequence to human SNX27 5′-AACCAGGTAATTGCATTTGAA-3′ and its inactive control 5′-CCATAATACAAG GTATAA-3′ were purchased from Qiagen (Valencia CA). Human embryonic kidney-293 (HEK-293) cells (CRL-1573) were from ATCC (Manassas, VA). HEK-293 cells passages 40–46 were grown in DMEM supplemented with 10% fetal bovine serum.
Nooh M.M., Mancarella S, & Bahouth S.W. (2016). Identification of Novel Transplantable GPCR Recycling Motif for Drug Discovery. Biochemical pharmacology, 120, 22-32.
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2

HEK-293 Cell Procurement from ATCC

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HEK-293 cells CRL-1573 were purchased from ATCC (https://www.atcc.org/products/crl-11268).

Cho N., Kontou G., Smalley J.L., Bope C., Dengler J., Montrose K., Deeb T.Z., Brandon N.J., Yamamoto T., Davies P.A., Giamas G, & Moss S.J. (2024). The brain-specific kinase LMTK3 regulates neuronal excitability by decreasing KCC2-dependent neuronal Cl− extrusion. iScience, 27(4), 109512.
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3

HEK 293 and THP-1 Cell Culture Protocol

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HEK 293 (CRL-1573™) cells were obtained from American Type Culture Collection (ATCC, Manassas, MDVA, USA) and cultured in Dulbecco’s modified Eagle’s medium (Gibco™, Waltham, MA, USA; 11965118) with 10% fetal bovine serum (Gibco™, 16000044) and 1% antibiotic-antimycotic (Gibco™, 15240062) in a humidified atmosphere in a 5% CO2 incubator at 37°C. THP-1 (TIB-202™) cells were obtained from ATCC and maintained in RPMI-1640 Medium (Gibco™, 21875034) containing 10% fetal bovine serum, 1% antibiotic-antimycotic and 0.05 mM 2-mercaptoethanol (Gibco™, 21985023). THP-1 cells were then stimulated with 50 nM phorbol myristate acetate (Sigma-Aldrich, St. Louis, MO, USA; P8139) for 24 h for induction of differentiation into macrophages.
Kim S.Y., Ban H.J., Lee S, & Jin H.J. (2022). Regulation of CIRP by genetic factors of SP1 related to cold sensitivity. Frontiers in Immunology, 13, 994699.
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4

Cell Culture Protocol for HEK293 and MEFs

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HEK293 (CRL-1573) cells were purchased from the American Type Culture Collection. Primary MEFs and HSFs (so-called F2S) were prepared as previously described (8 (link)).
Ao Y., Zhang J., Liu Z., Qian M., Li Y., Wu Z., Sun P., Wu J., Bei W., Wen J., Wu X., Li F., Zhou Z., Zhu W.G., Liu B, & Wang Z. (2019). Lamin A buffers CK2 kinase activity to modulate aging in a progeria mouse model.. Science Advances, 5(3), eaav5078.
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5

HEK293 Cell Cultivation for Reagent Purification

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HEK293 CRL-1573 cells were obtained from the American Type Culture Collection (Manassas, VA). The identity of these cells was not subsequently authenticated because the cells were only used for the purification of reagents rather than as experimental materials. The cells were grown in Dulbecco’s Modified Eagle Medium +10% Fetal Bovine Serum (Invitrogen, ThermoFisher Scientific) in the presence of 100 units/ml of penicillin and 100 μg/ml of streptomycin and a 1:100 dilution of the antimycotic Fungizone (Gibco, ThermoFisher Scientific).
Filter J.J., Williams B.C., Eto M., Shalloway D, & Goldberg M.L. (2017). Unfair competition governs the interaction of pCPI-17 with myosin phosphatase (PP1-MYPT1). eLife, 6, e24665.
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