P116 cells stably expressing wild type or mutant Zap-70 were grown in serum-free media for 4 hrs and then stimulated in culture for with 25 μg/ml Concanavalin A (Sigma-Aldrich) or mixed with equal number Raji cells alone or Raji cells and 100 ng/ml Staphylococcal enterotoxin D (SED; Toxin Technology) (Shapiro et al., 1998 (link)). Cell culture supernatants were collected after 24 hours and IL-2 assayed by ELISA with Human IL-2 ELISA Max Deluxe kit (BioLegend) using VersaMax Microplate Reader with SoftMax Pro Software (Molecular Devices).
Human il 2 elisa max deluxe kit
Manufactured by BioLegend
The Human IL-2 ELISA Max Deluxe kit is a laboratory equipment product designed for the quantitative measurement of human interleukin-2 (IL-2) in various sample types. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the IL-2 protein.
Automatically generated - may contain errors
2 protocols using human il 2 elisa max deluxe kit
1
Assaying IL-2 Production in Zap-70 Expressing Cells
2
Cytokine Secretion Quantification by ELISA
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IFN-γ, IL2, and IL12
secretion was measured by collecting the supernatant from the respective
cell-based assay and frozen at −20 °C prior to analysis
by ELISA. IFN-γ, IL2, and IL12 ELISAs were carried out using
the Human IFN-γ ELISA MAX Deluxe kit, Human IL2 ELISA MAX Deluxe
kit, and Human IL12 (p70) ELISA MAX Deluxe kit in accordance with
the manufacturer’s instructions (BioLegend).
secretion was measured by collecting the supernatant from the respective
cell-based assay and frozen at −20 °C prior to analysis
by ELISA. IFN-γ, IL2, and IL12 ELISAs were carried out using
the Human IFN-γ ELISA MAX Deluxe kit, Human IL2 ELISA MAX Deluxe
kit, and Human IL12 (p70) ELISA MAX Deluxe kit in accordance with
the manufacturer’s instructions (BioLegend).
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