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Inverted lsm780 microscope

Manufactured by Zeiss
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The Inverted LSM780 microscope is a high-performance confocal laser scanning microscope. It is designed for advanced imaging applications, providing high-resolution, multi-channel imaging capabilities. The core function of the Inverted LSM780 is to enable detailed and precise observations of biological samples through confocal laser scanning technology.

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Lab products found in correlation

Cd3 17a2, by Thermo Fisher Scientific (2 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (2 mentions) Ra3 6b2, by Thermo Fisher Scientific (2 mentions) Smz1270 stereo microscope, by Nikon (2 mentions) Superfrostplus adhesion glass, by Thermo Fisher Scientific (2 mentions) Plan apochromat 63 na 1.40 oil immersion objective, by Zeiss (2 mentions) Superfrostplus adhesion glass, by Nikon (2 mentions) Plan apochromat 20x 0.8 m27, by Zeiss (1 mentions)

3 protocols using inverted lsm780 microscope

1

Multimodal Imaging of Immune Cells

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For immunofluorescence, spleens were embedded and frozen in OCT, sectioned at 15 μm and mounted on SuperFrostPlus Adhesion glass (Thermo Fisher Scientific). Sections were dehydrated using silica beads, fixed with 4% paraformaldehyde for 10 min and washed with PBS. Samples were blocked using 5% normal goat serum for 2 h before staining. Samples were incubated with antibodies against B220 (RA3-6B2, eBioscience), CD3 (17A2, eBioscience) and F4/80 (BM8, Biolegend) diluted in 5% NGS for 2 h at room temperature in the dark. After staining, samples were washed with PBS at least three times. Samples were then mounted using ProLong Gold Antifade Mountant (Invitrogen) and imaged using an inverted LSM780 microscope (Carl Zeiss) and a plan apochromat 63× NA 1.40 oil-immersion objective (Carl Zeiss). For haematoxylin and eosin (H&E) staining, organs were collected and fixed in 10% formalin. Fixed samples were embedded in paraffin and sectioned at 10 μm, mounted on SuperFrostPlus Adhesion glass and stained using H&E. Mounted samples were imaged using a Nikon SMZ1270 Stereo Microscope. Imaging data were analysed using Fiji (ImageJ) software (NIH).
Tsui C., Kretschmer L., Rapelius S., Gabriel S.S., Chisanga D., Knöpper K., Utzschneider D.T., Nüssing S., Liao Y., Mason T., Torres S.V., Wilcox S.A., Kanev K., Jarosch S., Leube J., Nutt S.L., Zehn D., Parish I.A., Kastenmüller W., Shi W., Buchholz V.R, & Kallies A. (2022). MYB orchestrates T cell exhaustion and response to checkpoint inhibition. Nature, 609(7926), 354-360.
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2

Immunofluorescence and Histological Analysis

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For immunofluorescence, spleens were embedded and frozen in OCT, sectioned at 15 μm and mounted on SuperFrostPlus Adhesion glass (Thermo Fisher Scientific). Sections were dehydrated using silica beads, fixed with 4% paraformaldehyde for 10 min and washed with PBS. Samples were blocked using 5% normal goat serum for 2 h before staining. Samples were incubated with antibodies against B220 (RA3-6B2, eBioscience), CD3 (17A2, eBioscience) and F4/80 (BM8, Biolegend) diluted in 5% NGS for 2 h at room temperature in the dark. After staining, samples were washed with PBS at least three times. Samples were then mounted using ProLong Gold Antifade Mountant (Invitrogen) and imaged using an inverted LSM780 microscope (Carl Zeiss) and a plan apochromat 63× NA 1.40 oil-immersion objective (Carl Zeiss). For haematoxylin and eosin (H&E) staining, organs were collected and fixed in 10% formalin. Fixed samples were embedded in paraffin and sectioned at 10 μm, mounted on SuperFrostPlus Adhesion glass and stained using H&E. Mounted samples were imaged using a Nikon SMZ1270 Stereo Microscope. Imaging data were analysed using Fiji (ImageJ) software (NIH).
Tsui C., Kretschmer L., Rapelius S., Gabriel S.S., Chisanga D., Knöpper K., Utzschneider D.T., Nüssing S., Liao Y., Mason T., Torres S.V., Wilcox S.A., Kanev K., Jarosch S., Leube J., Nutt S.L., Zehn D., Parish I.A., Kastenmüller W., Shi W., Buchholz V.R, & Kallies A. (2022). MYB orchestrates T cell exhaustion and response to checkpoint inhibition. Nature, 609(7926), 354-360.
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3

Time-Lapse Imaging of Encapsulated Cells

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To maintain capsules in set positions for several days of time-lapse acquisitions, 20 - 25 capsules were selected 24 hours post cell encapsulation and embedded in 0.4% low-melting agarose (0.04 g in 10 mL) in a 35 mm MatTek glass-bottom dish. The agarose was left to solidify for 15 minutes and 2 mL of MEM containing no phenol red, supplemented with 1% (vol/vol) Penicillin-Streptomycin, 1% (vol/vol) non-essential amino acids (NEAA) 100X, 10% (vol/vol) FBS and 1% (vol/vol) GlutaMax were added. Live time-lapse confocal images of samples were obtained using inverted LSM780 microscope (Carl Zeiss) using the objective Plan-Apochromat 20x/0.8 M27 (FWD=0.55mm). During imaging, capsules were maintained at 37 °C with 5% CO2. For each capsule, 3D confocal Z-stacks with a range of 100 μm to 250 μm with 2 μm interval were acquired, and each capsule was imaged every 2. 5 – 3 h for 25 – 30 cycles using with definite focus (autofocus). For imaging of fixed samples, upright microscope LSM710 NLO was used with the objective W Plan-Apochromat 20x/1.0 DIC M27 75mm.
Trushko A., Di Meglio I., Merzouki A., Blanch-Mercader C., Abuhattum S., Guck J., Alessandri K., Nassoy P., Kruse K., Chopard B, & Roux A. (2020). Buckling of an Epithelium Growing under Spherical Confinement. Developmental Cell, 54(5), 655-668.e6.
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