PLG-PEMA nanoparticles were prepared using a single emulsion-solvent evaporation method. PLG with carboxylate end groups, a 50:50 d,l -lactide/glycolide ratio and inherent viscosity of 0.18 dL/g in hexafluoro-2-propanol was purchased from Lactel Absorbable Polymers (Birmingham, AL); PEMA (poly[ethylene-alt-maleic anhydride] was purchased from Polysciences, Inc., Warrington, PA. Two milliliters of 20% (w/v) PLG in dichloromethane (DCM) (Sigma, St. Louis, MO) was added dropwise to 4mL of 1% (w/v) aqueous PEMA, and the mixture was sonicated for 30 s at 100% amplitude using a Cole-Parmer CPX130 Ultrasonic Processor equipped with a Cole-Parmer CV 18 ultrasonic probe adapter and a Cole-Parmer 3 mm probe with stepped tip. Immediately after sonication, the emulsion was poured into 200 mL of 0.5% (w/v) aqueous PEMA under stirring on a Bellstir Multistir 9 magnetic stirrer. The resultant nanoparticles were stirred for 12 h to allow for the DCM to completely evaporate and were then washed three times in 0.1 M sodium carbonate-sodium bicarbonate buffer, pH 9.6. The particles were then resuspended in a 20 mL solution of 3% (w/v) aqueous d -mannitol and 4% (w/v) aqueous sucrose, gradually frozen to −80 °C, and lyophilized to dryness.
3 mm probe
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The 3 mm probe is a laboratory equipment designed for various measurements and applications. It features a compact 3 mm diameter tip and is suitable for a range of testing and analysis tasks. The core function of this probe is to provide accurate and reliable data collection in suitable environments.
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Preparation of PLG-PEMA Nanoparticles
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Synthesis of PLG-PEMA Nanoparticles
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PLG-PEMA nanoparticles were prepared using a single emulsion-solvent evaporation method. PLG with carboxylate end groups, a 50:50 d ,l -lactide/glycolide ratio and inherent viscosity of 0.18 dL/g in hexafluoro-2-propanol was purchased from Lactel Absorbable Polymers (Birmingham, AL); PEMA (poly[ethylene-alt-maleic anhydride] was purchased from Polysciences, Inc., Warrington, PA. Two milliliters of 20% (w/v) PLG in dichloromethane (DCM) (Sigma, St. Louis, MO) was added dropwise to 4 mL of 1% (w/v) aqueous PEMA, and the mixture was sonicated for 30 s at 100% amplitude using a Cole-Parmer CPX130 Ultrasonic Processor equipped with a Cole-Parmer CV 18 ultrasonic probe adapter and a Cole-Parmer 3 mm probe with stepped tip. Immediately after sonication, the emulsion was poured into 200 mL of 0.5% (w/v) aqueous PEMA under stirring on a Bellstir Multistir 9 magnetic stirrer. The resultant nanoparticles were stirred for 12 h to allow for the DCM to completely evaporate and were then washed three times in 0.1 M sodium carbonate-sodium bicarbonate buffer, pH 9.6. The particles were then resuspended in a 20 mL solution of 3% (w/v) aqueous d -mannitol and 4% (w/v) aqueous sucrose, gradually frozen to −80 °C, and lyophilized to dryness.
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