5′ stranded libraries were constructed using NEB Next Multiplex Small RNA Library Prep. Resulting cDNA was PCR amplified and gel purified prior to sequencing. Libraries were subjected to 75bp single end RNA Seq using NextSeq500 Illumina sequencer, High Output Kit v2.5 (75 Cycles) (Next Generation Sequencing Facility, Faculty of Medicine, University of Leeds).
Next multiplex small rna library prep
Manufactured by New England Biolabs
The Next Multiplex Small RNA Library Prep is a lab equipment product designed for the preparation of small RNA libraries. It enables the multiplexed preparation of multiple small RNA samples simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using next multiplex small rna library prep
1
Small RNA Sequencing Library Prep
2
Ribosome Footprinting and Small RNA Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RA was added to SH-SY5Y cells 3 days prior to harvesting. Ribosome footprints were subjected to rRNA depletion (Illumina RiboZero rRNA removal kit).
' stranded libraries were constructed using NEB Next Multiplex Small RNA Library Prep.
Resulting cDNA was PCR amplified and gel purified prior to sequencing. Libraries were subjected to 75bp single end RNA Seq using NextSeq500 Illumina sequencer, High Output Kit v2.5 (75 Cycles) (Next Generation Sequencing Facility, Faculty of Medicine, University of Leeds).
' stranded libraries were constructed using NEB Next Multiplex Small RNA Library Prep.
Resulting cDNA was PCR amplified and gel purified prior to sequencing. Libraries were subjected to 75bp single end RNA Seq using NextSeq500 Illumina sequencer, High Output Kit v2.5 (75 Cycles) (Next Generation Sequencing Facility, Faculty of Medicine, University of Leeds).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!