MCF‐7 and Bcap‐37 cells were transfected with miR‐148a‐3p mimics and negative control miR (miR‐NC) (Sigma Aldrich) by Lipofectamine 2000. MiR‐148a‐3p antagomir was transfected with Lipofectamine RNAi MAX Kit. After 48 h for transfection, the efficiency of transfection was determined by quantitative reverse transcription (qRT)‐polymerase chain reaction (PCR).
Mir nc
Manufactured by Merck Group
Sourced in United States
MiR-NC is a laboratory equipment product from Merck Group. It is designed for the detection and quantification of microRNA (miRNA) expression levels in biological samples. The core function of MiR-NC is to provide a reliable and efficient platform for miRNA analysis, enabling researchers to gain insights into gene regulation and cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Pcdna3.1 vector, by Sangon (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Si nc, by Sangon (1 mentions)
Scramble sirna, by Merck Group (1 mentions)
Biocoat control cell culture inserts, by Corning (1 mentions)
Si nc, by Merck Group (1 mentions)
Lipofectamine rnaimax kit, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by Merck Group (1 mentions)
Mir 26b mimics, by Merck Group (1 mentions)
5 protocols using mir nc
1
Transfection of miR-148a-3p in Breast Cancer Cells
2
Modulating SNHG12 and miR-195 in Cells
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SNHG12 small interfering RNA (siRNA) and the non-targeting control sequence (si-NC) were synthesized by Sangon Biotech Co., Ltd. The SNHG12 expressing plasmid was constructed by inserting the full-length human SNHG12 sequence into pcDNA3.1 vector from Sangon Biotech Co., Ltd. MiR-195 mimics (#HMI0320) and the scramble miRNA control (miR-NC) (#HMI0321) were purchased from Sigma-Aldrich (Sigma, USA). Cell transfections were performed using Lipofectamine 2000 reagent (Invitrogen) and at appropriate concentrations.
3
Investigating miR-584-5p Effects on Clonogenic and Migration Abilities
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MB cells were transfected with miR-584-5p, miR-NC, scramble-siRNA, or target-siRNA (Sigma) for 24 h, harvested, and subjected to long-term clonogenic and migration assays62 (link). For the long-term clonogenic assays, 1000 cells/well were reseeded in six-well plates for an additional 7–10 days until colonies were visible. Colonies were fixed with 4% paraformaldehyde and stained with 1% crystal violet. For the transwell migration assay, 100,000 cells were reseeded in Corning BioCoat Control Cell Culture Inserts with 8.0-µm PET Membrane in serum-free medium. Cells were allowed to migrate toward complete media for 24 h before fixation with 4% paraformaldehyde and staining with 1% crystal violet.
4
TP53TG1 Overexpression and Silencing
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The TP53TG1 overexpression vector (pcDNA-TP53TG1) was commercially constructed by GenePharma (Shanghai, China) and empty pcDNA vector was used as the negative control. All miRNA mimics (miR-18a mimics, miR-NC), miRNA inhibitors (anti-miR-NC, anti-miR-18a), and si-RNAs (si-NC, si-TP53TG1#1 and si-TP53TG1#2) were designed and synthesized by Sigma-Aldrich (St. Louis, MO, USA). All oligonucleotides and plasmids were transfected into A549 or A549/DDP cells using the Lipofectamine 3000 Transfection Reagent (Invitrogrn) according to the manufacturer’s instructions.
5
Lipofectamine-Mediated Cell Transfection
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Cell transfection was performed by using the Lipofectamine RNAi MAX kit (invitrogen) according to the instructions. Of which, miR-26b mimics, miR-26b inhibitor and corresponding negative controls (miR-NC and inhibitor NC) were purchased from Sigma Aldrich.
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