Milli q filtered

Polycaprolactone (PCL, M_n ≈ 10,000 Da, determined by gel permeation chromatography) and polysorbate 80 (T80) were purchased from Sigma-Aldrich (Sintra, Portugal) and used without further purification. The reagents and solutions used for cell-based assays were described in [8 (link)] and are detailed in the Supplementary Materials. EndoGRO Media was acquired from Merck (Cambridge, MA, USA) and rat tail collagen type I (low viscosity) was purchased from Cultrex. Other reagents were obtained from Sigma-Aldrich (Sintra, Portugal). The water used was Milli-Q filtered (Millipore, Burlington, MA, USA).

The human lung adenocarcinoma A549 cell line was purchased from the American Type Culture Collection (ATCC, Manassas, USA). Calf serum, Dulbecco’s modified Eagle’s medium (DMEM) and trypsin were purchased from Thermo Scientific (Waltham, USA). The Cell Counting Kit-8 (CCK-8) was obtained from the Beyot Institute of Biotechnology (Haimen, Jiangsu, China). All other chemicals were obtained as analytical grade reagents from Sigma (St. Louis, MO, USA) or Sinopharm Chemical Reagent (SCRC, Shanghai, China). Chemicals used in 2DE were purchased from Amresco (Ohio, USA) or GE (Piscataway, USA). Water used in experiments was Millipore Milli-Q filtered (Bedford, MA, USA) at a resistivity greater than or equal to 18.25 Ω·cm. Immobilized pH gradient (IPG) strips were purchased from GE (Piscataway, USA). Culture dishes, 6-well plates and 96-well plates were supplied by Costar (Cambridge, USA). Polyvinylidene difluoride (PVDF) membranes were from Millipore (Bedford, MA, USA). Primary antibodies, the MTF-1 rabbit anti-human polyclonal (C-terminus) antibody (LS-C31406/26273), anti-metallothionein antibody (ab12228), anti-SLC39A1 antibody (ab105416), SLC30A1 antibody (NBP1-56273) were purchased. Secondary antibodies consisting of anti-rabbit lgG-HRP (sc-2004) and goat anti-mouse lgG-HRP (sc-2005) were also purchased.