Bovine pancreatic trypsin inhibitor

Manufactured by Merck Group

Sourced in United Kingdom

Bovine pancreatic trypsin inhibitor (BPTI) is a protein that functions as a protease inhibitor, specifically inhibiting the serine protease trypsin. It is derived from the pancreas of bovine animals. BPTI is used in various applications, such as biochemical research and pharmaceutical development, where it serves to regulate and control the activity of trypsin.

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Trypsin (3740 citations) Trypsin inhibitor (281 citations) Bovine trypsin (50 citations) Bovine pancreatic trypsin (22 citations)

2 protocols using bovine pancreatic trypsin inhibitor

Preparation of BPTI and beta casein

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Bovine pancreatic trypsin inhibitor (BPTI) and beta casein were purchased from Sigma Aldrich, Irvine, UK; 1,3-dicyanobenzene was obtained from the Waters Corporation, Wilmslow, UK (Analytical Standards and Reagents); ammonium acetate was purchased from Fisher Scientific, Loughborough, UK; 1-2 mg/mL stock solutions of BPTI and beta casein were prepared in 50 mM ammonium acetate, pH 7. Prior to being used for mass spectrometry analysis, BPTI was diluted to 10 μM, whereas beta casein was desalted with biospin-6 columns (BioRad, Hecules, California, USA) then diluted before use.

Jhingree J.R., Bellina B., Pacholarz K.J, & Barran P.E. (2017). Charge Mediated Compaction and Rearrangement of Gas-Phase Proteins: A Case Study Considering Two Proteins at Opposing Ends of the Structure-Disorder Continuum. Journal of the American Society for Mass Spectrometry, 28(7), 1450-1461.

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Peptide Synthesis and Purification Protocol

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Gold(III) chloride trihydrate, cetyltrimethylammonium bromide (CTAB), hydroquinone, sodium borohydride, silver nitrate, trifluoroacetic acid (TFA), 2,2′-(ethylenedioxy)diethanethiol, thioanisole (EODT), anisole, piperidine, trypsin from porcine pancreas (T4799), and bovine pancreatic trypsin inhibitor (BPTI) were purchased from Sigma-Aldrich. Fmoc-amino acids, Fmoc-Gly-Wang resin, and HBTU were received from Aapptec (Louisville, KY, USA). Diisopropylethylamine and ethyl ether anhydrous were purchased from Fisher Scientific. GCKGCG, GGKGGG, and GGKGCG were either purchased from Genscript Inc. (New Jersey, USA) or synthesized. Pooled human urine was collected from Innovative research (Novi, MI).

Creyer M.N., Jin Z., Moore C., Yim W., Zhou J, & Jokerst J.V. (2021). Modulation of Gold Nanorod Growth via the Proteolysis of Dithiol Peptides for Enzymatic Biomarker Detection. ACS applied materials & interfaces, 13(38), 45236-45243.

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