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Dgh00

Manufactured by R&D Systems
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Sourced in United Kingdom

The DGH00 is a piece of laboratory equipment designed for general use in research and experimental settings. It serves as a tool for performing various tasks and procedures as required by the user's specific research needs.

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Lab products found in correlation

Dg100, by R&D Systems (3 mentions) Anti human ttr, by Agilent Technologies (1 mentions) Somascan assay platform, by SomaLogic (1 mentions) Dve00, by R&D Systems (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Dgb300, by R&D Systems (1 mentions) Kge007, by R&D Systems (1 mentions) Dbb00, by R&D Systems (1 mentions)

4 protocols using dgh00

1

Quantification of Serum Biomarkers

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Serum samples were analyzed with commercially available ELISA kits for GH (DGH00, R&D Systems, Minneapolis, MN), IGF-1 (DG100, R&D Systems, Minneapolis, MN), IGFBP-3 (DBG300, R&D Systems, Minneapolis, MN), and GLP-2 (YK141, Yanaihara Institute, Shizuoka, Japan) according to manufacturer instructions.
Rowan M.P., Beckman D.J., Rizzo J.A., Isbell C.L., White C.E., Cohn S.M, & Chung K.K. (2016). Elevations in growth hormone and glucagon-like peptide-2 levels on admission are associated with increased mortality in trauma patients. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 24, 119.
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2

GH Levels in Glioblastoma Cells

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Plasma GH levels of patients from ONT tissue bank for whom tumour levels of GHR mRNA had been determined were measured using ELISA (DGH00, R&D Systems, Abingdon, UK). The same assay was used to measure GH secreted by GBM cell cultures. Briefly, 500 000 cells were plated in 6‐well plates in serum‐free B27/GF/FGF‐containing DMEM/F12 medium. Forty‐five hours later, cell suspension (neurospheres) was harvested, centrifuged, and supernatant was isolated and frozen until use.
Verreault M., Segoviano Vilchis I., Rosenberg S., Lemaire N., Schmitt C., Guehennec J., Royer‐Perron L., Thomas J., Lam T.T., Dingli F., Loew D., Ducray F., Paris S., Carpentier C., Marie Y., Laigle‐Donadey F., Rousseau A., Pigat N., Boutillon F., Bielle F., Mokhtari K., Frank S.J., de Reyniès A., Hoang‐Xuan K., Sanson M., Goffin V, & Idbaih A. (2022). Identification of growth hormone receptor as a relevant target for precision medicine in low‐EGFR expressing glioblastoma. Clinical and Translational Medicine, 12(7), e939.
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3

Plasma Proteome Profiling for Bariatric Surgery

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Plasma proteome profiling was performed using the high-throughput DNA aptamer-based SOMAscan assay platform (SomaLogic, Inc.)78 (link). The abundance of 1129 proteins (enriched for extracellular proteins) was quantified as relative fluorescent units (RFU), normalized, calibrated, and log2-transformed. Samples were available at baseline, 3, 12, 24, and 36 month time points for 19, 19, 19, 15, and 14 participants in the RYGB arm, and for 19, 19, 16, 10, and 9 participants in the DWM arm (Supplementary Data 1).
Selected proteomic data were validated by ELISA in a subset of fasting plasma samples, including IGFBP2 (22-BP2HU-E01, ALPCO, NH), CNDP1 (F34010, LifeSpan Biosciences, WA), growth hormone (DGH00, R&D Systems, MN), and total IGF-1 (DG100, R&D Systems, MN). RBP4 and TTR were assayed by quantitative western blotting using polyclonal anti-human RBP4 (Dako) and anti-human TTR (Dako) with standard curves of purified human RBP4 or TTR (Sigma) on each blot79 (link). Changes per individual over time were tested for positive correlation to corresponding SOMAscan changes with a one-sided test of Pearson correlation.
Dreyfuss J.M., Yuchi Y., Dong X., Efthymiou V., Pan H., Simonson D.C., Vernon A., Halperin F., Aryal P., Konkar A., Sebastian Y., Higgs B.W., Grimsby J., Rondinone C.M., Kasif S., Kahn B.B., Foster K., Seeley R., Goldfine A., Djordjilović V, & Patti M.E. (2021). High-throughput mediation analysis of human proteome and metabolome identifies mediators of post-bariatric surgical diabetes control. Nature Communications, 12, 6951.
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4

Temporal Dynamics of Biomarkers in PRP Therapy

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A blood sample of approximately 20 mL was taken from the brachial vein of the untreated limb at 1 h before and at 1, 2, and 7 days after PRP injection. To mitigate the confounding effects of diurnal variation and the metabolic effects of diet and acute bouts of exercise, blood was drawn at precisely the same time each morning between 8 and 10 am and at least 6 h after eating or training.
The blood specimen was centrifuged at 3000 × 3 for 10 min and then stored at -80°C until enzyme-linked immunosorbent assay (ELISA) assessment. The concentrations of target proteins were assessed using a Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA) following the manufacturer's instructions. Results were calculated by interpolation from a standard curve established from graded concentrations of GH (DGH00, R&D Systems), IGF-1 (DG100, R&D Systems), IGFBP-3 (DGB300, R&D Systems), PDGF-BB (DBB00, R&D Systems), VEGF (DVE00, R&D Systems), and substance P(SP) (KGE007, R&D Systems).
Kuo S.J., Chou W.Y., Hsu C.C., Chang-Chien G.P., Lin S.F., Siu K.K., Tsai T.C., Ko J.Y, & Sun Y.C. (2020). Systemic effects of platelet-rich plasma local injection on serum and urinary anabolic metabolites: A prospective randomized study. The Chinese journal of physiology, 63(6).
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