Tgf beta 1 duoset elisa

The expression levels of EGFR, IGF-IR, and TGFβ1 were encountered in serum samples from NSCLC male patients. Healthy subjects were detected using ELISA, with the Human EGFR DuoSet ELISA (R&D System, Minneapolis, MN, USA, cat No. DY231), Ancillary Reagent Kit 2 (R&D System, cat No. DY008), IGFIR DuoSet ELISA (R&D System, cat No. DY 391), Ancillary Reagent Kit 3 (R&D System, cat No. DY240-05), TGF beta 1 DuoSet ELISA, and Ancillary Reagent Kit 1 (R&D System, cat No. DY 007).

mLN and SILP cells were prepared as described above before incubating with 50μg/ml T. spiralis antigen for 24 hours in media (RPMI-1640, 10% FCS, 100U/ml Pen/strp, 5%NEAA, L-glutamine and HEPES, 0.05 mM β-mercaptoethanol (SIGMA)). Cell-free supernatants were analysed for cytokine production via cytometric bead array (BD) or paired ELISA antibodies (anti-IFNγ, clone XMG1.2 and R4-6A2; anti-IL-13, clone eBio13A and eBio1316H; anti-IL-4, clone 11B1and BVD6-2462, anti-IL-17 clone eBio17CK15A5 and eBio17B7; (eBioscience)). For TGFβ analysis samples were acid-activated prior to detection on a mouse TGF-beta 1 DuoSet ELISA (R and D Systems).