Blood samples were collected into several 8 mL CPT Mononuclear Cell Preparation Tubes-sodium citrate (BD #362761) and stored at 4°C for up to 24 hours until preparation. Peripheral blood mononuclear cells (PBMC) were enriched by centrifuging the tubes at 1,700xg for 20 minutes at 25°C. After removing a 1 mL aliquot of plasma, the mononuclear cells were poured into a 50 mL conical tube and washed twice with MACS buffer (PBS containing 0.5% fetal bovine serum and 2 mM EDTA). The washed cell pellet was then suspended in 50 μl MACS buffer containing human Fc block (BD #564219) prior to staining.
Cpt mononuclear cell preparation tubes sodium citrate
Manufactured by BD
The CPT Mononuclear Cell Preparation Tubes-sodium citrate are laboratory equipment used for the separation and isolation of mononuclear cells from whole blood samples. The tubes contain a density gradient medium and an anticoagulant, sodium citrate, to facilitate the separation process.
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2 protocols using cpt mononuclear cell preparation tubes sodium citrate
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Isolation of PBMC from Whole Blood
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Isolation of PBMCs from Whole Blood
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Peripheral blood was collected into several 8 mL CPT Mononuclear Cell Preparation Tubes Sodium Citrate (BD, 362761) and stored at 4°C for up to 24 hours until processing. Tubes were then centrifuged at 1700g for 20 minutes at 25°C, and 1 mL aliquot of plasma was collected. The remaining PBMCs were removed by aspiration, washed twice with MACS buffer (PBS containing 0.5% fetal bovine serum and 2 mM EDTA), and suspended in 50 μL MACS buffer containing human Fc block (BD, 564219) prior to staining.
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