Anti human mouse granzyme b

Manufactured by BioLegend

Anti‐human/mouse Granzyme B is a recombinant protein that binds to and detects Granzyme B, a serine protease found in the cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells. It plays a key role in cell-mediated immune responses.

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Granzyme B (61 citations) Anti-granzyme B (13 citations) FITC anti-human/mouse Granzyme B (5 citations) FITC anti-human/mouse Granzyme B Antibody (4 citations) Anti-human Granzyme B (3 citations) Anti-mouse/human Granzyme B (clone GB11, (2 citations) Anti-human/mouse granzyme B Antibody (GB11) (2 citations)

2 protocols using anti human mouse granzyme b

Murine Immune Cell Phenotyping

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Conjugated mAbs anti‐mouse CD45 (30‐F11), CD3ε (17A2), H‐2K^b (AF6‐88.5), H‐2K^d (SF1‐1.1), NKG2D (CX5), FasL (MFL3), TRAIL (N2B2), DNAM‐1 (TX42.1), Ly49D (4E5), CD25 (PC61), Perforin (eBioOMAK‐D), Sca‐1 (D7), PD‐1 (29F.1A12), 2B4 (eBio244F4), KLRG1 (2F1), CD11b (M1/70), CD11c (N418), CD27 (LG.3A10), Thy1.2 (30‐H12), CD62L (MEL‐14), CD44 (IM7), NKp46 (29A1.4), NK1.1 (PK136), CD4 (RM4‐5), CD8α (53‐6.7), IFN‐γ (XMG1.2), Eomes (Dan11mag), T‐bet (O4‐46), Blimp‐1 (5E7), CD107a (1D4B), Foxp3 (NRRF‐30), CD16/32‐Fc blocking (93), and anti‐human/mouse Granzyme B (GB11) were purchased from BioLegend, eBioscience, and BD Pharmingen. Intracellular staining was done using the Foxp3 staining buffer set (eBioscience). DAPI (Life technologies) or fixable viability dye eFluor 450 or eFluor 506 (eBioscience) was used to exclude dead cells. Sample acquisition was done on a BD LSR Fortessa (BD Biosciences) using BD FACS Diva software (BD Biosciences) and analyzed using FlowJo (Tree Star).

Hüber C.M., Doisne J, & Colucci F. (2015). IL‐12/15/18‐preactivated NK cells suppress GvHD in a mouse model of mismatched hematopoietic cell transplantation. European Journal of Immunology, 45(6), 1727-1735.

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Tumor Immunotherapy Protocol

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Anti-mouse Tim-3 (clone: RMT3-23), anti-mouse PD-1 (clone: J43), anti-mouse Lag-3 (clone: C9B7W), Rat IgG isotype control (clone: 2A3) and polyclonal American hamster IgG were purchased from Bioxcell company for tumor therapy. For Flow cytometry, anti-mouse CD45 (clone: 30-F11), anti-mouse CD4 (clone: GK1.5), anti-mouse CD8a (clone: 53–6.7), anti-mouse Foxp3 (MF23), anti-mouse γδTCR (clone: GL3), anti-mouse CD103 (clone: M290), anti-mouse Tim-3 (clone: 5D12), anti-mouse Lag-3 (clone: C9B7W), anti-mouse Lag-3 (clone: C9B7W), anti-mouse CD206 (clone: MR5D3), anti-mouse CD62L (clone: MEL-14), anti-mouse CD44(clone: IM7), anti-mouse OX40 (OX-86), anti-mouse IL7R (clone: SB/199), anti-mouse GITR (clone:DTA-1) and anti-mouse CD11b (clone: M1/70) were purchased from BD Bioscience. anti-mouse Ki67(clone: SolA15), IFN-γ (clone: XMG1.2), CD11b (M1/70) were purchased from ebioscience. anti-mouse B220 (clone: RA3-6B2), anti-mouse CD8a (clone: 53–6.7), anti-human/mouse Granzyme B (clone: GB11), anti-mouse Granzyme A (clone: 3G8.5), anti-mouse MHC II (clone: M5/114.15.2), anti-mouse Gr-1 (clone: RB6-8C5), anti-mouse CD24 (clone: M1/69), anti-mouse F4/80 (clone: BM8) and anti-mouse NK1.1 (clone: PK136) were purchased from Biolegend. Mitotracker Deep Red FM labeling kit was purchased from Invitrogen. Ghost 510 and Zombie NIR dye was purchased from Tonbo Biosciences and Biolegend.

Yang M., Du W., Yi L., Wu S., He C., Zhai W., Yue C., Sun R., Menk A.V., Delgoffe G.M., Jiang J, & Lu B. (2020). Checkpoint molecules coordinately restrain hyperactivated effector T cells in the tumor microenvironment. Oncoimmunology, 9(1), 1708064.

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